细胞生物学
诱导多能干细胞
软骨内骨化
软骨
软骨细胞
细胞外基质
生物
再生医学
成骨细胞
干细胞
解剖
胚胎干细胞
体外
遗传学
基因
作者
Shireen R. Lamandé,Elizabeth S. Ng,Trevor L. Cameron,Louise H. W. Kung,Lisa Sampurno,Lynn Rowley,Jinia Lilianty,Yudha Nur Patria,Tayla Stenta,Eric Hanssen,Katrina M. Bell,Ritika Saxena,Kathryn S. Stok,Edouard G. Stanley,Andrew G. Elefanty,John F. Bateman
标识
DOI:10.1073/pnas.2211510120
摘要
Chondrocytes and osteoblasts differentiated from induced pluripotent stem cells (iPSCs) will provide insights into skeletal development and genetic skeletal disorders and will generate cells for regenerative medicine applications. Here, we describe a method that directs iPSC-derived sclerotome to chondroprogenitors in 3D pellet culture then to articular chondrocytes or, alternatively, along the growth plate cartilage pathway to become hypertrophic chondrocytes that can transition to osteoblasts. Osteogenic organoids deposit and mineralize a collagen I extracellular matrix (ECM), mirroring in vivo endochondral bone formation. We have identified gene expression signatures at key developmental stages including chondrocyte maturation, hypertrophy, and transition to osteoblasts and show that this system can be used to model genetic cartilage and bone disorders.
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