相互作用体
内膜系统
网格蛋白
拟南芥
复印机
信号转导衔接蛋白
网格蛋白接合器蛋白
高尔基体
细胞生物学
内吞循环
生物
蛋白质靶向
转运蛋白
拟南芥
内吞作用
囊泡转运蛋白
膜蛋白
小泡
分泌途径
生物化学
信号转导
基因
突变体
内质网
受体
膜
作者
Peng Wang,Wei Siao,Xiuyang Zhao,Deepanksha Arora,Ren Wang,Dominique Eeckhout,Jelle Van Leene,Rahul Kumar,Anaxi Houbaert,Nancy De Winne,Evelien Mylle,Michaël Vandorpe,Ruud A. Korver,Christa Testerink,Kris Gevaert,Steffen Vanneste,Geert De Jaeger,Daniël Van Damme,Eugenia Russinova
出处
期刊:Nature plants
[Springer Nature]
日期:2023-01-12
卷期号:9 (2): 355-371
被引量:11
标识
DOI:10.1038/s41477-022-01328-2
摘要
Adaptor protein (AP) complexes are evolutionarily conserved vesicle transport regulators that recruit coat proteins, membrane cargoes and coated vesicle accessory proteins. As in plants endocytic and post-Golgi trafficking intersect at the trans-Golgi network, unique mechanisms for sorting cargoes of overlapping vesicular routes are anticipated. The plant AP complexes are part of the sorting machinery, but despite some functional information, their cargoes, accessory proteins and regulation remain largely unknown. Here, by means of various proteomics approaches, we generated the overall interactome of the five AP and the TPLATE complexes in Arabidopsis thaliana. The interactome converged on a number of hub proteins, including the thus far unknown adaptin binding-like protein, designated P34. P34 interacted with the clathrin-associated AP complexes, controlled their stability and, subsequently, influenced clathrin-mediated endocytosis and various post-Golgi trafficking routes. Altogether, the AP interactome network offers substantial resources for further discoveries of unknown endomembrane trafficking regulators in plant cells.
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