细胞生物学
骨重建
运输机
糖
化学
新陈代谢
碳水化合物代谢
生物化学
生物
内分泌学
基因
作者
Pei Ying Ng,A. Ribet,Qiang Guo,Benjamin H. Mullin,Jamie Tan,Euphemie Landao‐Bassonga,Sébastien Stephens,Kai Chen,Jinbo Yuan,Laila N. Abudulai,Maike Bollen,Edward T. T. T. Nguyen,Jasreen Kular,J. M. Papadimitriou,Kent Søe,Rohan D. Teasdale,Jiake Xu,Robert G. Parton,Hiroshi Takayanagi,Nathan J. Pavlos
标识
DOI:10.1038/s41467-023-36484-2
摘要
Osteoclasts are giant bone-digesting cells that harbor specialized lysosome-related organelles termed secretory lysosomes (SLs). SLs store cathepsin K and serve as a membrane precursor to the ruffled border, the osteoclast's 'resorptive apparatus'. Yet, the molecular composition and spatiotemporal organization of SLs remains incompletely understood. Here, using organelle-resolution proteomics, we identify member a2 of the solute carrier 37 family (Slc37a2) as a SL sugar transporter. We demonstrate in mice that Slc37a2 localizes to the SL limiting membrane and that these organelles adopt a hitherto unnoticed but dynamic tubular network in living osteoclasts that is required for bone digestion. Accordingly, mice lacking Slc37a2 accrue high bone mass owing to uncoupled bone metabolism and disturbances in SL export of monosaccharide sugars, a prerequisite for SL delivery to the bone-lining osteoclast plasma membrane. Thus, Slc37a2 is a physiological component of the osteoclast's unique secretory organelle and a potential therapeutic target for metabolic bone diseases.
科研通智能强力驱动
Strongly Powered by AbleSci AI