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Marked cell-type-specific differences in glycosylation of human interleukin-6

糖基化 生物 分子生物学 糖蛋白 氨基酸 生物化学 成纤维细胞 肽序列 细胞培养 体外 基因 遗传学
作者
Lauren T. May,James E. Shaw,Ashwani Khanna,J B Zabriskie,P B Sehgal
出处
期刊:Cytokine [Elsevier BV]
卷期号:3 (3): 204-211 被引量:37
标识
DOI:10.1016/1043-4666(91)90018-9
摘要

Human interleukin-6 (IL-6) secreted by cytokine- or endotoxin-induced fibroblasts, monocytes, keratinocytes, endometrial stromal cells, and endothelial cells, when analyzed under denaturing and reducing conditions, consists of a set of differentially modified phosphoglyco-proteins of molecular mass in the range from 23 to 30 kD (a set of at least three O-glycosylated 23- to 25-kD species and a set of at least three N- and O-glycosylated 28- to 30-kD species). The 23- to 25-kD and 28- to 30-kD fibroblast-derived IL-6 species have been separately purified to homogeneity with the use of a combination of lectin and immunoaffinity chromatography. Glycosidase digestion experiments on such purified preparations confirmed that almost all human fibroblast-derived IL-6 species were O-glycosylated; additionally, the 28- to 30-kD species were N-glycosylated. Amino acid sequencing revealed that the major amino terminus in the fibroblast-derived 23- to 25-kD O-glycosylated IL-6 was at Ala28 whereas the major amino terminus in the 28- to 30-kD N- and O-glycosylated IL-6 was at Val30, suggesting that targeting of newly synthesized IL-6 polypeptides into the two different processing pathways in fibroblasts may be keyed to differences in the signal peptide cleavage site. Unexpectedly, IL-6 “constitutively” secreted by the Epstein-Barr virus (EBV)-infected human and primate (tamarin) B-cell lines designated sfBJAB and sfBT, respectively, consisted of a major apparently unglycosylated 21-kD species and a minor 25-kD N-glycosylated species. Similarly, human IL-6 secreted by Sf9 insect cells infected with a baculovirus expression vector for human IL-6 consisted of a 21-kD unglycosylated species (amino terminus at Ala28) and a 25-kD N-glycosylated species. In contrast, IL-6 secreted by the EBV-transformed human B-cell line KH consisted exclusively of the 28- to 30-kD N- and O-glycosylated species. Taken together, the present data highlight the marked differences in the glycosylation patterns of IL-6 polypeptides secreted by different human cell types and help reconcile previous disparate descriptions of natural human IL-6 proteins.
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