清晨好,您是今天最早来到科研通的研友!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您科研之路漫漫前行!

Marked cell-type-specific differences in glycosylation of human interleukin-6

糖基化 生物 分子生物学 糖蛋白 氨基酸 生物化学 成纤维细胞 肽序列 细胞培养 体外 基因 遗传学
作者
Lauren T. May,James E. Shaw,Ashwani Khanna,J B Zabriskie,P B Sehgal
出处
期刊:Cytokine [Elsevier BV]
卷期号:3 (3): 204-211 被引量:37
标识
DOI:10.1016/1043-4666(91)90018-9
摘要

Human interleukin-6 (IL-6) secreted by cytokine- or endotoxin-induced fibroblasts, monocytes, keratinocytes, endometrial stromal cells, and endothelial cells, when analyzed under denaturing and reducing conditions, consists of a set of differentially modified phosphoglyco-proteins of molecular mass in the range from 23 to 30 kD (a set of at least three O-glycosylated 23- to 25-kD species and a set of at least three N- and O-glycosylated 28- to 30-kD species). The 23- to 25-kD and 28- to 30-kD fibroblast-derived IL-6 species have been separately purified to homogeneity with the use of a combination of lectin and immunoaffinity chromatography. Glycosidase digestion experiments on such purified preparations confirmed that almost all human fibroblast-derived IL-6 species were O-glycosylated; additionally, the 28- to 30-kD species were N-glycosylated. Amino acid sequencing revealed that the major amino terminus in the fibroblast-derived 23- to 25-kD O-glycosylated IL-6 was at Ala28 whereas the major amino terminus in the 28- to 30-kD N- and O-glycosylated IL-6 was at Val30, suggesting that targeting of newly synthesized IL-6 polypeptides into the two different processing pathways in fibroblasts may be keyed to differences in the signal peptide cleavage site. Unexpectedly, IL-6 “constitutively” secreted by the Epstein-Barr virus (EBV)-infected human and primate (tamarin) B-cell lines designated sfBJAB and sfBT, respectively, consisted of a major apparently unglycosylated 21-kD species and a minor 25-kD N-glycosylated species. Similarly, human IL-6 secreted by Sf9 insect cells infected with a baculovirus expression vector for human IL-6 consisted of a 21-kD unglycosylated species (amino terminus at Ala28) and a 25-kD N-glycosylated species. In contrast, IL-6 secreted by the EBV-transformed human B-cell line KH consisted exclusively of the 28- to 30-kD N- and O-glycosylated species. Taken together, the present data highlight the marked differences in the glycosylation patterns of IL-6 polypeptides secreted by different human cell types and help reconcile previous disparate descriptions of natural human IL-6 proteins.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
负责以山完成签到 ,获得积分10
45秒前
胖小羊完成签到 ,获得积分10
46秒前
Akim应助Demi_Ming采纳,获得10
49秒前
51秒前
sunshine完成签到 ,获得积分10
58秒前
美满的冬卉完成签到 ,获得积分10
58秒前
Axs完成签到,获得积分10
1分钟前
完美世界应助科研通管家采纳,获得10
1分钟前
megumin完成签到,获得积分10
1分钟前
TEY完成签到 ,获得积分10
1分钟前
jiangqin123完成签到 ,获得积分10
1分钟前
菠萝包完成签到 ,获得积分10
1分钟前
2分钟前
2分钟前
Demi_Ming发布了新的文献求助10
2分钟前
winne完成签到,获得积分10
2分钟前
2分钟前
wanci应助李小猫采纳,获得10
2分钟前
山楂完成签到,获得积分10
2分钟前
2分钟前
2分钟前
和谐乌龟发布了新的文献求助10
2分钟前
和谐乌龟完成签到,获得积分10
2分钟前
ttyhtg完成签到,获得积分10
2分钟前
2分钟前
bkagyin应助萧萧采纳,获得10
2分钟前
阿巴完成签到 ,获得积分10
3分钟前
李小猫发布了新的文献求助10
3分钟前
Hello应助科研通管家采纳,获得10
3分钟前
drhwang完成签到,获得积分10
3分钟前
研究生完成签到 ,获得积分10
3分钟前
3分钟前
3分钟前
4分钟前
4分钟前
4分钟前
谦让寻凝完成签到 ,获得积分10
5分钟前
土豆··发布了新的文献求助20
5分钟前
5分钟前
淡淡醉波wuliao完成签到 ,获得积分0
5分钟前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
Immigrant Incorporation in East Asian Democracies 600
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3968504
求助须知:如何正确求助?哪些是违规求助? 3513318
关于积分的说明 11167297
捐赠科研通 3248697
什么是DOI,文献DOI怎么找? 1794414
邀请新用户注册赠送积分活动 875030
科研通“疑难数据库(出版商)”最低求助积分说明 804652