两亲性
化学
共聚物
胶束
磁共振造影剂
纳米颗粒
环氧乙烷
甲基丙烯酸酯
阿霉素
超顺磁性
乳状液
生物物理学
核化学
材料科学
有机化学
水溶液
纳米技术
聚合物
物理
磁化
量子力学
磁场
生物
医学
外科
化疗
作者
Kangning Zhu,Zhicheng Deng,Guhuan Liu,Jinming Hu,Shiyong Liu
出处
期刊:Macromolecules
[American Chemical Society]
日期:2017-01-25
卷期号:50 (3): 1113-1125
被引量:61
标识
DOI:10.1021/acs.macromol.6b02162
摘要
The development of stimuli-responsive magnetic resonance imaging (MRI) contrast agents that can selectively enhance imaging contrasts at pathological sites is of potential use in clinical diagnosis. Herein, a T2-type MRI contrast agent with synergistically photoregulated enhanced MRI contrast and drug release was achieved by coassembly of superparamagnetic iron oxide nanoparticles (SPIONs) and doxorubicin (DOX) with amphiphilic block copolymer assemblies. Photosensitive amphiphilic diblock copolymers, poly(ethylene oxide)-b-poly(2-((((2-nitrobenzyl)oxy)carbonyl)amino)ethyl methacrylate) (PEO-b-PNBOC), were synthesized through reversible addition–fragmentation chain transfer (RAFT) polymerizations. The resulting block copolymers were coassembled with hydrophobic oleic acid (OA)-stabilized SPIONs and DOX via an oil-in-water (O/W) emulsion and a subsequent solvent evaporation procedure, resulting in the formation of DOX/SPION coloaded hybrid nanovectors. The as-assembled hybrid nanovectors exhibited retarded DOX release and weak T2 relaxivity (r2) prior to UV-irradiation. However, upon UV-irradiation, the hybrid nanovectors underwent cross-linking and a hydrophobic-to-hydrophilic transition within the cores, thereby selectively triggering DOX release and elevating T2 relaxivities. In vitro DOX release results revealed approximately 85% of DOX was released within 10 h under 20 min UV-irradiation, and this was in sharp contrast with less than 5% of DOX release without UV-irradiation. The selective DOX release under UV-irradiation showed significantly increased cytotoxicity toward HepG2 cells. Meanwhile, the r2 of UV-irradiated nanovectors exhibited 4.5- and 1.9-fold increases as compared to cetyltrimethylammonium bromide (CTAB)-stabilized monodispersed SPIONs and nonirradiated hybrid nanovectors. Moreover, there was a linear correlation between the r2 changes and cumulative DOX release extents, enabling instantaneously visualizing the DOX release by the MRI technique. Further, we demonstrated that the cellular internalization efficiency of the coloaded hybrid nanovectors increased by 2.7-fold in the presence of an external magnet. The magnetically guided cellular uptake, triggered release profile, and enhanced MRI contrast characteristics may presage potential applications as a new generation of theranostic platform.
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