Identification and characterization of genes related to cellulolytic activity in basidiomycetes

presented the highest cellulolytic halo to mycelial growth radius ratio, followed by L. crinitus.Four genes were amplified in the L. edodes strain, whereas three and one genes were isolated from L. crinitus and S. commune, respectively.The cel6B gene (L.edodes) presented the conserved domain glyco_hydro_6 and characterized as cellobiohydrolase gene.The results of this study contribute to the existing knowledge on cellulases in basidiomycetes, and serve as a basis for future studies on the expression of these genes and the characterization of the catalytic activity of these enzymes.This allows for better utilization of these fungi in degrading vegetal fibers from agro-industrial residues and in other biotechnological applications.