摘要
Journal of Food BiochemistryVolume 41, Issue 3 e12352 FULL ARTICLE Characterization and antioxidant activities of polysaccharides extracted from enzymatic hydrolysate of Ginkgo biloba leaves Chang-Wei Zhang, Chang-Wei Zhang Institute of Chemical Industry of Forest Products, CAF, Nanjing, 210042 Jiangsu, China National Engineering Laboratory for Biomass Chemical Utilization, Nanjing, 210042 Jiangsu, China Key and Open Laboratory on Forest Chemical Engineering, SFA, Nanjing, 210042 Jiangsu, China Key Laboratory of Biomass Energy and Material, Nanjing, 210042 Jiangsu, ChinaSearch for more papers by this authorCheng-Zhang Wang, Corresponding Author Cheng-Zhang Wang wangczlhs@sina.com Institute of Chemical Industry of Forest Products, CAF, Nanjing, 210042 Jiangsu, China National Engineering Laboratory for Biomass Chemical Utilization, Nanjing, 210042 Jiangsu, China Key and Open Laboratory on Forest Chemical Engineering, SFA, Nanjing, 210042 Jiangsu, China Key Laboratory of Biomass Energy and Material, Nanjing, 210042 Jiangsu, China Institute of New Technology of Forestry, CAF, Beijing, 100091 ChinaCorrespondence Cheng-Zhang Wang, Institute of Chemical Industry of Forest Products, CAF, Nanjing 210042, Jiangsu, China. Email: wangczlhs@sina.comSearch for more papers by this authorRan Tao, Ran Tao Institute of Chemical Industry of Forest Products, CAF, Nanjing, 210042 Jiangsu, China National Engineering Laboratory for Biomass Chemical Utilization, Nanjing, 210042 Jiangsu, China Key and Open Laboratory on Forest Chemical Engineering, SFA, Nanjing, 210042 Jiangsu, China Key Laboratory of Biomass Energy and Material, Nanjing, 210042 Jiangsu, ChinaSearch for more papers by this author Chang-Wei Zhang, Chang-Wei Zhang Institute of Chemical Industry of Forest Products, CAF, Nanjing, 210042 Jiangsu, China National Engineering Laboratory for Biomass Chemical Utilization, Nanjing, 210042 Jiangsu, China Key and Open Laboratory on Forest Chemical Engineering, SFA, Nanjing, 210042 Jiangsu, China Key Laboratory of Biomass Energy and Material, Nanjing, 210042 Jiangsu, ChinaSearch for more papers by this authorCheng-Zhang Wang, Corresponding Author Cheng-Zhang Wang wangczlhs@sina.com Institute of Chemical Industry of Forest Products, CAF, Nanjing, 210042 Jiangsu, China National Engineering Laboratory for Biomass Chemical Utilization, Nanjing, 210042 Jiangsu, China Key and Open Laboratory on Forest Chemical Engineering, SFA, Nanjing, 210042 Jiangsu, China Key Laboratory of Biomass Energy and Material, Nanjing, 210042 Jiangsu, China Institute of New Technology of Forestry, CAF, Beijing, 100091 ChinaCorrespondence Cheng-Zhang Wang, Institute of Chemical Industry of Forest Products, CAF, Nanjing 210042, Jiangsu, China. Email: wangczlhs@sina.comSearch for more papers by this authorRan Tao, Ran Tao Institute of Chemical Industry of Forest Products, CAF, Nanjing, 210042 Jiangsu, China National Engineering Laboratory for Biomass Chemical Utilization, Nanjing, 210042 Jiangsu, China Key and Open Laboratory on Forest Chemical Engineering, SFA, Nanjing, 210042 Jiangsu, China Key Laboratory of Biomass Energy and Material, Nanjing, 210042 Jiangsu, ChinaSearch for more papers by this author First published: 06 February 2017 https://doi.org/10.1111/jfbc.12352Citations: 7 Funding information: Jiangsu Province Forestry Engineering Sanxin Engineering project, Grant/Award Number: LYSX[2014]09; International Science and Technology Cooperation Program of China, Grant/Award Number: 2014DFR31300 Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract Three high purified polysaccharide monomers (GBP11, GBP22, and GBP33) were obtained from enzymatic hydrolysate of Ginkgo biloba leave using DEAE-Sepharose Fast Flow column and Sephadex G-100 column, and their basic structural characterization were investigated by utilizing high-performance liquid chromatography, high-performance ion chromatography, and Infrared Spectroscopy. In addition, antioxidant activities of related polysaccharides were also evaluated. The results showed that the molecular weight of GBP11, GBP22, and GBP33 were 1.944 × 103 KDa, 5.167 × 103 KDa, and 45.98 KDa, singly, and they were mainly made up of glucose, galactose, arabinose, and rhamnose with a molar ratio of 62.2:1.98:3.84:1.24, 3.57:68.0:4.67:0.36 and 5.34:48.8:1.70:8.97, respectively. Moreover, GBP22, GBP, and GBP11 demonstrated the strongest free radical scavenging activity on DPPH, ABTS, and superoxide anion, showing that they have potential application value on the functional food and cosmetic industry. Practical applications Enzymatic hydrolysate of Ginkgo biloba leaves includes lots of carbohydrate, it will cause eutrophication and environment pollution if it is directly poured out. This research indicates that carbohydrate extracted from enzymatic hydrolysate of Ginkgo biloba leaves are made up of different polysaccharide monomers with various molecular weight and monosaccharide composition. Moreover, these polysaccharide monomers possess appreciating antioxidant activities. Therefore, this research could lay a foundation for the high-value application of polysaccharides in functional food and cosmetic industry, and provide theoretical reference for the treatment of waste water. Citing Literature Volume41, Issue3June 2017e12352 RelatedInformation