生物素
聚合物
链霉亲和素
生物素化
亲和素
光漂白
荧光
生物传感器
化学
聚合
荧光显微镜
组合化学
生物物理学
纳米技术
材料科学
生物化学
有机化学
物理
量子力学
生物
作者
Paula Relógio,Maël Bathfield,Zofia Haftek-Terreau,Mariana Beija,Arnaud Favier,Marie-Josèphe Giraud-Panis,Franck D’Agosto,Bernard Mandrand,José Paulo S. Farinha,Marie‐Thérèse Charreyre,J. M. G. Martinho
出处
期刊:Polymer Chemistry
[The Royal Society of Chemistry]
日期:2013-01-01
卷期号:4 (10): 2968-2968
被引量:37
摘要
Many biosensing and imaging systems use fluorescence detection. We present the synthesis of biotin-end-functionalized highly fluorescent water-soluble polymers for potential use in biotin–avidin systems. Statistical polymers of N-acryloylmorpholine (NAM) and N-acryloxysuccinimide (NAS) were prepared by RAFT polymerization using a biotinylated chain transfer agent that ensured 95% end-functionalization of the chains. They were further labeled with a lucifer yellow (LY) dye, yielding 7 to 62 LY fluorophores per polymer chain. The resulting polymers exhibited reduced fluorescence self-quenching, with 7- to 43-fold higher brightness than free LY dye. In addition, they featured low pH sensitivity and very good photobleaching resistance. Moreover, we showed that a more extended polymer conformation was beneficial to the binding of the terminal biotin with streptavidin. This work paves the way for the development of polymers for signal amplification in biosensing assays, labeling of biotin-receptors at cell surfaces in some cancer studies, labeling of antibodies and microscopy imaging purposes.
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