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Nanoparticle shell structural cues drive in vitro transport properties, tissue distribution and brain accessibility in zebrafish

纳米载体 体内分布 材料科学 磷酰胆碱 生物物理学 PEG比率 体内 纳米颗粒 蛋白质吸附 涂层 聚乙二醇 表面改性 血脑屏障 纳米技术 背景(考古学) 聚合物 体外 化学 生物化学 生物 财务 古生物学 中枢神经系统 物理化学 神经科学 经济 复合材料 生物技术
作者
Jean‐Michel Rabanel,Jimmy Faivre,Charlotte Zaouter,Shunmoogum A. Patten,Xavier Banquy,Charles Ramassamy
出处
期刊:Biomaterials [Elsevier]
卷期号:277: 121085-121085 被引量:7
标识
DOI:10.1016/j.biomaterials.2021.121085
摘要

Zwitterion polymers with strong antifouling properties have been suggested as the prime alternative to polyethylene glycol (PEG) for drug nanocarriers surface coating. It is believed that PEG coating shortcomings, such as immune responses and incomplete protein repellency, could be overcome by zwitterionic polymers. However, no systematic study has been conducted so far to complete a comparative appraisal of PEG and zwitterionic-coating effects on nanoparticles (NPs) stealthness, cell uptake, cell barrier translocation and biodistribution in the context of nanocarriers brain targeting. Core-shell polymeric particles with identical cores and a shell of either PEG or poly(2-methacryloyloxyethyl phosphorylcholine (PMPC) were prepared by impinging jet mixer nanoprecipitation. NPs with similar size and surface potential were systematically compared using in vitro and in vivo assays. NPs behavior differences were rationalized based on their protein-particles interactions. PMPC-coated NPs were significantly more endocytosed by mouse macrophages or brain resident macrophages compared to PEGylated NPs but exhibited the remarkable ability to cross the blood-brain barrier in in vitro models. Nanoscale flow cytometry assays showed significantly more adsorbed proteins on PMPC-coated NPs than PEG-coated NPs. In vivo, distribution in zebrafish larvae, showed a strong propensity for PMPC-coated NPs to adhere to the vascular endothelium, while PEG-coated NPs were able to circulate for a longer time and escape the bloodstream to penetrate deep into the cerebral tissue. The stark differences between these two types of particles, besides their similarities in size and surface potential, points towards the paramount role of surface chemistry in controlling NPs fate likely via the formation of distinct protein corona for each coating.
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