角膜
炎症
医学
共焦显微镜
CD11c公司
角膜炎症
全身炎症
全身给药
CpG寡核苷酸
病理
免疫学
体内
眼科
细胞生物学
生物
表型
基因表达
生物技术
DNA甲基化
基因
生物化学
作者
Haihan Jiao,Cecilia Naranjo Golborne,Samantha J. Dando,Paul G. McMenamin,Laura E Downie,Holly R. Chinnery
标识
DOI:10.1080/09273948.2019.1646775
摘要
Purpose We report novel differences in mouse corneal DC morphology and density during local and systemic inflammation.Methods Local inflammation was induced by topical application of saline or TLR9 agonist CpG-ODN on abraded C57BL6J mouse corneas. Systemic inflammation was induced by intraperitoneal injection of lipopolysaccharide (LPS) in CD11c-YFP mice. Corneal epithelial DCs from uninjured, injured and contralateral eyes were analysed by confocal microscopy.Results Following local CpG delivery on the injured cornea, the DC density and size increased in both central and peripheral regions. Contralateral uninjured eyes displayed enlarged DC morphology in the central cornea compared to naïve cohorts. After systemic LPS, the size of DCs in the central cornea was lower at 2 hours, returning to baseline after 24 hours.Conclusions Corneal DCs respond differently in terms of shape and distribution during local and systemic inflammation. These features can serve as in vivo indicators in ocular and systemic diseases.
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