稳健性(进化)
分析物
三级四极质谱仪
质谱法
样品制备
色谱法
计算机科学
稳健性测试
工艺工程
电喷雾电离
可靠性工程
化学
选择性反应监测
串联质谱法
工程类
人工智能
生物化学
基因
模糊逻辑
出处
期刊:Elsevier eBooks
[Elsevier]
日期:2016-11-11
卷期号:: 37-62
被引量:26
标识
DOI:10.1016/b978-0-12-800871-3.00003-1
摘要
The principles and optimization of sample preparation protocols commonly used in clinical LC-MS/MS are described and compared in this chapter. Although the practices reviewed here are more broadly applicable, in this chapter “LC-MS/MS” refers to reverse-phase LC, UPLC, or UHPLC used with electrospray (ESI), atmospheric pressure chemical ionization (APCI), and triple quadrupole (QqQ) quantitative mass analysis. In general, those protocols that are more labor intensive, complex and/or expensive are also more effective at concentrating analytes and removing matrix than simpler and cheaper protocols. Matrix removal is beneficial because injecting less matrix/sample typically yields more robust methods and longer maintenance-free intervals for the LC-MS/MS instrument. Goals for method performance, such as precision and quantitation limit, are well defined in clinical chemistry, but there is less agreement on what constitutes method robustness. The challenge for clinical laboratories using LC-MS/MS is to select sample preparation protocols that deliver not only acceptable method performance but also the desired cost/reportable test, practicality and robustness. Strategies for evaluating robustness during validation—at best an incomplete proxy for robustness in production—are presented and metrics for quantifying robustness in production are proposed.
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