已入深夜,您辛苦了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!祝你早点完成任务,早点休息,好梦!

Spinosyn A and Its Derivative Inhibit Colorectal Cancer Cell Growth via the EGFR Pathway

小桶 生物 细胞生长 细胞培养 结直肠癌 癌症研究 分子生物学 基因 基因表达 癌症 遗传学 基因本体论
作者
Kunjian Peng,Zizheng Zou,Jijia Li,Yuanzhu Xie,Zhengnan Ming,Ting Jiang,Wensong Luo,Xiyuan Hu,Yuan Nie,Ling Chen,Tiao Luo,Ting Peng,Da‐You Ma,Suyou Liu,Zhi-Yong Luo
出处
期刊:Journal of Natural Products [American Chemical Society]
卷期号:86 (9): 2111-2121 被引量:2
标识
DOI:10.1021/acs.jnatprod.3c00276
摘要

Spinosyn A (SPA), derived from a soil microorganism, Saccharopolyspora spinosa, and its derivative, LM2I, has potential inhibitory effects on a variety of cancer cells. However, the effects of SPA and LM2I in inhibiting the growth of human colorectal cancer cells and the molecular mechanisms underlying these effects are not fully understood. Cell viability was tested by using a 3-(4,5-dimethylthiazol-2-yl-)-2,5-diphenyltetrazolium bromide (MTT) assay and a colony formation assay. On the basis of the IC50 values of SPA and LM2I in seven colorectal cancer (CRC) cell lines, sensitive (HT29 and SW480) and insensitive (SW620 and RKO) cell lines were screened. The GSE2509 and GSE10843 data sets were used to identify 69 differentially expressed genes (DEGs) between sensitive and insensitive cell lines. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, and protein-protein interactions (PPI) were performed to elucidate the molecular mechanisms of the DEGs. The hub gene of the DEGs was detected by Western blot analysis and verified using the CRISPR/Cas9 system. Our data indicate that SPA and its derivative LM2I have significant antiproliferative activity in seven colorectal cancer cell lines and colorectal xenograft tumors. On the basis of bioinformatics analysis, it was demonstrated that epidermal growth factor receptor (EGFR) was the hub gene of the DEGs and was associated with the inhibitory effects of SPA and LM2I in CRC cell lines. The study also revealed that SPA and LM2I inhibited the EGFR pathway in vitro and in vivo.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
chengymao完成签到,获得积分10
7秒前
CodeCraft应助lplmid采纳,获得10
8秒前
xiaozhao完成签到 ,获得积分10
17秒前
20秒前
爱科研的小周完成签到 ,获得积分10
23秒前
三更笔舞完成签到,获得积分10
26秒前
YANGLan完成签到,获得积分10
29秒前
小二郎应助科研通管家采纳,获得10
31秒前
完美世界应助科研通管家采纳,获得10
31秒前
汉堡包应助科研通管家采纳,获得10
31秒前
小蘑菇应助科研通管家采纳,获得10
31秒前
31秒前
於艳血完成签到,获得积分10
32秒前
传奇3应助优美的背包采纳,获得10
33秒前
思源应助YOG采纳,获得10
33秒前
仰山雪完成签到 ,获得积分10
34秒前
wenlong完成签到 ,获得积分10
35秒前
38秒前
ai发布了新的文献求助10
38秒前
39秒前
42秒前
44秒前
阿童木完成签到,获得积分10
47秒前
YOG发布了新的文献求助10
48秒前
48秒前
damie完成签到 ,获得积分10
52秒前
Ava应助Mu采纳,获得10
53秒前
泥泥完成签到,获得积分20
54秒前
CodeCraft应助野性的凌瑶采纳,获得10
55秒前
压缩完成签到 ,获得积分10
56秒前
重要问芙brk完成签到,获得积分10
57秒前
Shirley发布了新的文献求助30
1分钟前
1分钟前
小小旭呀应助忧虑的羊采纳,获得10
1分钟前
科研通AI2S应助sam采纳,获得10
1分钟前
1分钟前
1分钟前
1分钟前
1分钟前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
2-Acetyl-1-pyrroline: an important aroma component of cooked rice 500
A real-time energy management strategy based on fuzzy control and ECMS for PHEVs 400
Handbook on People's China (1957) 400
2024 Medicinal Chemistry Reviews 400
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3189894
求助须知:如何正确求助?哪些是违规求助? 2839133
关于积分的说明 8023069
捐赠科研通 2502078
什么是DOI,文献DOI怎么找? 1336196
科研通“疑难数据库(出版商)”最低求助积分说明 637804
邀请新用户注册赠送积分活动 605852