Spinosyn A and Its Derivative Inhibit Colorectal Cancer Cell Growth via the EGFR Pathway

小桶 生物 细胞生长 细胞培养 结直肠癌 癌症研究 分子生物学 基因 基因表达 癌症 遗传学 基因本体论
作者
Kunjian Peng,Zizheng Zou,Jijia Li,Yuanzhu Xie,Zhengnan Ming,Ting Jiang,Wensong Luo,Xiyuan Hu,Yuan Nie,Ling Chen,Tiao Luo,Ting Peng,Da‐You Ma,Suyou Liu,Zhi-Yong Luo
出处
期刊:Journal of Natural Products [American Chemical Society]
卷期号:86 (9): 2111-2121 被引量:2
标识
DOI:10.1021/acs.jnatprod.3c00276
摘要

Spinosyn A (SPA), derived from a soil microorganism, Saccharopolyspora spinosa, and its derivative, LM2I, has potential inhibitory effects on a variety of cancer cells. However, the effects of SPA and LM2I in inhibiting the growth of human colorectal cancer cells and the molecular mechanisms underlying these effects are not fully understood. Cell viability was tested by using a 3-(4,5-dimethylthiazol-2-yl-)-2,5-diphenyltetrazolium bromide (MTT) assay and a colony formation assay. On the basis of the IC50 values of SPA and LM2I in seven colorectal cancer (CRC) cell lines, sensitive (HT29 and SW480) and insensitive (SW620 and RKO) cell lines were screened. The GSE2509 and GSE10843 data sets were used to identify 69 differentially expressed genes (DEGs) between sensitive and insensitive cell lines. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, and protein-protein interactions (PPI) were performed to elucidate the molecular mechanisms of the DEGs. The hub gene of the DEGs was detected by Western blot analysis and verified using the CRISPR/Cas9 system. Our data indicate that SPA and its derivative LM2I have significant antiproliferative activity in seven colorectal cancer cell lines and colorectal xenograft tumors. On the basis of bioinformatics analysis, it was demonstrated that epidermal growth factor receptor (EGFR) was the hub gene of the DEGs and was associated with the inhibitory effects of SPA and LM2I in CRC cell lines. The study also revealed that SPA and LM2I inhibited the EGFR pathway in vitro and in vivo.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
彭于晏应助虚幻的蛋挞采纳,获得10
1秒前
田一发布了新的文献求助10
1秒前
崔家荣完成签到,获得积分10
1秒前
tanbao完成签到,获得积分10
2秒前
单纯芮完成签到,获得积分20
3秒前
3秒前
3秒前
Refuel完成签到,获得积分10
4秒前
老邱发布了新的文献求助10
4秒前
华仔应助义气的一德采纳,获得10
4秒前
4秒前
皛鑫森淼焱垚完成签到,获得积分10
4秒前
爱吃烤苕皮完成签到,获得积分10
5秒前
Owen应助无异常采纳,获得10
5秒前
leaf完成签到 ,获得积分10
6秒前
lany应助西音采纳,获得10
6秒前
yefeng完成签到,获得积分10
6秒前
ztlooo完成签到,获得积分10
6秒前
jayus完成签到,获得积分20
7秒前
7秒前
maorongfu456完成签到,获得积分10
8秒前
8秒前
8秒前
Negan完成签到,获得积分10
8秒前
缥缈的背包完成签到,获得积分10
8秒前
8秒前
答铃关注了科研通微信公众号
9秒前
wangdi应助himsn采纳,获得10
9秒前
单纯芮发布了新的文献求助10
9秒前
9秒前
jayus发布了新的文献求助10
10秒前
11秒前
Green完成签到,获得积分10
11秒前
可可窦完成签到,获得积分10
12秒前
柴六斤发布了新的文献求助10
12秒前
赘婿应助沉静的曼荷采纳,获得10
12秒前
13秒前
zcxlsy6关注了科研通微信公众号
13秒前
智慧少女不头秃完成签到,获得积分10
13秒前
isabelwy发布了新的文献求助10
13秒前
高分求助中
Evolution 3rd edition 1500
保险藏宝图 1000
Lire en communiste 1000
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 700
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 700
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3183813
求助须知:如何正确求助?哪些是违规求助? 2834035
关于积分的说明 7997276
捐赠科研通 2496339
什么是DOI,文献DOI怎么找? 1332081
科研通“疑难数据库(出版商)”最低求助积分说明 636519
邀请新用户注册赠送积分活动 603705