Spinosyn A and Its Derivative Inhibit Colorectal Cancer Cell Growth via the EGFR Pathway

小桶 生物 细胞生长 细胞培养 结直肠癌 癌症研究 分子生物学 基因 基因表达 癌症 遗传学 基因本体论
作者
Kunjian Peng,Zizheng Zou,Jijia Li,Yuanzhu Xie,Zhengnan Ming,Ting Jiang,Wensong Luo,Xiyuan Hu,Yuan Nie,Ling Chen,Tiao Luo,Ting Peng,Da‐You Ma,Suyou Liu,Zhi-Yong Luo
出处
期刊:Journal of Natural Products [American Chemical Society]
卷期号:86 (9): 2111-2121 被引量:2
标识
DOI:10.1021/acs.jnatprod.3c00276
摘要

Spinosyn A (SPA), derived from a soil microorganism, Saccharopolyspora spinosa, and its derivative, LM2I, has potential inhibitory effects on a variety of cancer cells. However, the effects of SPA and LM2I in inhibiting the growth of human colorectal cancer cells and the molecular mechanisms underlying these effects are not fully understood. Cell viability was tested by using a 3-(4,5-dimethylthiazol-2-yl-)-2,5-diphenyltetrazolium bromide (MTT) assay and a colony formation assay. On the basis of the IC50 values of SPA and LM2I in seven colorectal cancer (CRC) cell lines, sensitive (HT29 and SW480) and insensitive (SW620 and RKO) cell lines were screened. The GSE2509 and GSE10843 data sets were used to identify 69 differentially expressed genes (DEGs) between sensitive and insensitive cell lines. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, and protein-protein interactions (PPI) were performed to elucidate the molecular mechanisms of the DEGs. The hub gene of the DEGs was detected by Western blot analysis and verified using the CRISPR/Cas9 system. Our data indicate that SPA and its derivative LM2I have significant antiproliferative activity in seven colorectal cancer cell lines and colorectal xenograft tumors. On the basis of bioinformatics analysis, it was demonstrated that epidermal growth factor receptor (EGFR) was the hub gene of the DEGs and was associated with the inhibitory effects of SPA and LM2I in CRC cell lines. The study also revealed that SPA and LM2I inhibited the EGFR pathway in vitro and in vivo.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
小肖完成签到 ,获得积分10
刚刚
1秒前
LAN0528发布了新的文献求助10
1秒前
2秒前
2秒前
堇年完成签到 ,获得积分10
3秒前
兰贵人发布了新的文献求助10
6秒前
任嘉嘉发布了新的文献求助10
6秒前
7秒前
寒冷苞络完成签到,获得积分10
7秒前
wangrswjx应助lidapan采纳,获得10
10秒前
夏天完成签到 ,获得积分10
11秒前
科研通AI2S应助BENpao123采纳,获得10
11秒前
用心听发布了新的文献求助10
12秒前
劲秉应助杜玉涵采纳,获得10
12秒前
昏睡的眼神完成签到 ,获得积分10
12秒前
NexusExplorer应助liujy采纳,获得10
14秒前
任嘉嘉完成签到,获得积分20
15秒前
大个应助bliss采纳,获得10
15秒前
16秒前
用心听完成签到,获得积分20
18秒前
21秒前
未月初二完成签到,获得积分10
21秒前
荣誉完成签到,获得积分10
21秒前
开放以南发布了新的文献求助10
21秒前
烟火会翻滚完成签到,获得积分10
22秒前
23秒前
23秒前
未月初二发布了新的文献求助10
24秒前
25秒前
曾经可乐完成签到 ,获得积分10
26秒前
不配.应助清新的静枫采纳,获得10
28秒前
28秒前
蓝冰完成签到,获得积分10
29秒前
归海神刀发布了新的文献求助10
31秒前
31秒前
珍珍完成签到 ,获得积分10
32秒前
平常的静枫完成签到 ,获得积分10
33秒前
幸福大白发布了新的文献求助50
33秒前
Yimi发布了新的文献求助10
36秒前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
ALA生合成不全マウスでの糖代謝異常の分子機構解析 520
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
2-Acetyl-1-pyrroline: an important aroma component of cooked rice 500
A real-time energy management strategy based on fuzzy control and ECMS for PHEVs 400
Handbook on People's China (1957) 400
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3189115
求助须知:如何正确求助?哪些是违规求助? 2838517
关于积分的说明 8020058
捐赠科研通 2501350
什么是DOI,文献DOI怎么找? 1335573
科研通“疑难数据库(出版商)”最低求助积分说明 637620
邀请新用户注册赠送积分活动 605656