Spinosyn A and Its Derivative Inhibit Colorectal Cancer Cell Growth via the EGFR Pathway

小桶 生物 细胞生长 细胞培养 结直肠癌 癌症研究 分子生物学 基因 基因表达 癌症 遗传学 基因本体论
作者
Kunjian Peng,Zizheng Zou,Jijia Li,Yuanzhu Xie,Zhengnan Ming,Ting Jiang,Wensong Luo,Xiyuan Hu,Yuan Nie,Ling Chen,Tiao Luo,Ting Peng,Da‐You Ma,Suyou Liu,Zhi-Yong Luo
出处
期刊:Journal of Natural Products [American Chemical Society]
卷期号:86 (9): 2111-2121 被引量:2
标识
DOI:10.1021/acs.jnatprod.3c00276
摘要

Spinosyn A (SPA), derived from a soil microorganism, Saccharopolyspora spinosa, and its derivative, LM2I, has potential inhibitory effects on a variety of cancer cells. However, the effects of SPA and LM2I in inhibiting the growth of human colorectal cancer cells and the molecular mechanisms underlying these effects are not fully understood. Cell viability was tested by using a 3-(4,5-dimethylthiazol-2-yl-)-2,5-diphenyltetrazolium bromide (MTT) assay and a colony formation assay. On the basis of the IC50 values of SPA and LM2I in seven colorectal cancer (CRC) cell lines, sensitive (HT29 and SW480) and insensitive (SW620 and RKO) cell lines were screened. The GSE2509 and GSE10843 data sets were used to identify 69 differentially expressed genes (DEGs) between sensitive and insensitive cell lines. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, and protein-protein interactions (PPI) were performed to elucidate the molecular mechanisms of the DEGs. The hub gene of the DEGs was detected by Western blot analysis and verified using the CRISPR/Cas9 system. Our data indicate that SPA and its derivative LM2I have significant antiproliferative activity in seven colorectal cancer cell lines and colorectal xenograft tumors. On the basis of bioinformatics analysis, it was demonstrated that epidermal growth factor receptor (EGFR) was the hub gene of the DEGs and was associated with the inhibitory effects of SPA and LM2I in CRC cell lines. The study also revealed that SPA and LM2I inhibited the EGFR pathway in vitro and in vivo.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
陶醉怜容完成签到,获得积分10
刚刚
1秒前
1秒前
孤独的匕完成签到,获得积分10
1秒前
winfree完成签到,获得积分10
1秒前
3秒前
852应助微笑念寒采纳,获得30
3秒前
ccl发布了新的文献求助20
4秒前
SiDi发布了新的文献求助150
5秒前
cqcqcq完成签到 ,获得积分10
6秒前
王小胖发布了新的文献求助10
6秒前
xiaotan完成签到 ,获得积分10
6秒前
孤独的匕发布了新的文献求助10
8秒前
8秒前
微笑语风发布了新的文献求助10
8秒前
9秒前
11秒前
小缪发布了新的文献求助10
11秒前
科研通AI2S应助明日复明日采纳,获得10
13秒前
13秒前
13秒前
Ava应助快乐的一只小跳蛙采纳,获得10
13秒前
14秒前
无花果应助cytoy采纳,获得10
14秒前
搜集达人应助姬昌采纳,获得10
15秒前
郭淳完成签到,获得积分10
16秒前
直率惜儿完成签到,获得积分10
16秒前
Joanna发布了新的文献求助10
17秒前
mgr完成签到,获得积分10
18秒前
18秒前
科研通AI2S应助搞怪烨伟采纳,获得10
18秒前
18秒前
万能图书馆应助zhlh采纳,获得10
19秒前
xilingang发布了新的文献求助10
20秒前
王小胖完成签到,获得积分20
20秒前
开心友儿完成签到,获得积分10
20秒前
22秒前
22秒前
搜集达人应助科研通管家采纳,获得10
23秒前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
Evolution 3rd edition 1500
保险藏宝图 1000
Lire en communiste 1000
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 700
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 700
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3184892
求助须知:如何正确求助?哪些是违规求助? 2835197
关于积分的说明 8003970
捐赠科研通 2497684
什么是DOI,文献DOI怎么找? 1333062
科研通“疑难数据库(出版商)”最低求助积分说明 636748
邀请新用户注册赠送积分活动 604174