无菌的
细菌
制浆造纸工业
对抗
干腐病
接种
松木
微观世界
降级(电信)
生物
植物
化学
食品科学
园艺
生态学
工程类
电信
生物化学
受体
遗传学
作者
Julia Embacher,Susanne Zeilinger,Sigrid Neuhauser,Martín Kirchmair
出处
期刊:Bio-protocol
[Bio-Protocol]
日期:2023-01-01
卷期号:13 (19)
标识
DOI:10.21769/bioprotoc.4837
摘要
Mixed communities of fungi and bacteria have been shown to be more efficient in degrading wood than fungi alone. Some standardised protocols for quantification of the wood decay ability of fungi have been developed (e.g., DIN V ENV 12038:2002 as the legal standard to test for the resistance of wood against wood-destroying basidiomycetes in Germany). Here, we describe a step-by-step protocol developed from the official standard DIN V ENV12038 to test combinations of bacteria and fungi for their combined wood degradation ability. Equally sized wood blocks are inoculated with wood decay fungi and bacterial strains. Axenic controls allow the analysis of varying degradation rates via comparison of the wood dry weights at the end of the experiments. This protocol provides new opportunities in exploration of inter- and intra-kingdom interactions in the wood-related environment and forms the basis for microcosm experiments. Key features • Quantification of wood decay ability of mixed cultures. • Allows testing if fungi are more efficient in degrading wood when bacteria are present.
科研通智能强力驱动
Strongly Powered by AbleSci AI