Functionalized nanofibers mat prepared through thiol–ene “click” reaction as solid phase extraction adsorbent for simultaneous detection of florfenicol and paracetamol residues in milk

Due to the significant differences in physical and chemical properties of various veterinary drugs, sample pretreatment is still the bottleneck of simultaneous detection of multiple veterinary drug residues. In order to achieve quantitative determination of two different types of veterinary drug residues (florfenicol and paracetamol) in milk, 1-allyl-3-methylimidazolium chloride (AmimCl) functionalized nanofibers mat (AmimCl-NFsM) was prepared through thiol-ene "click" reaction and applied as a new solid phase extraction adsorbent. The preparation parameters were systematically investigated and optimized through dynamic adsorption experiments. The developed method achieved significant extraction and purification efficiency, which was attributed to the multiple adsorption mechanisms of AmimCl-NFsM. Under the optimal condition, the established method showed low detection limit (1.25–2.90 ng/mL), high precision (RSDs ≤ 12.6 %) and good recovery (76.4 %–96.1 % with RSDs ≤ 5.23 %). The obtained results demonstrated the practical application value of the established method in food safety field.