生物正交化学
黑色素瘤
微泡
小泡
循环肿瘤细胞
点击化学
化学
细胞外小泡
外体
脂质体
生物物理学
适体
纳米技术
细胞生物学
癌症研究
生物化学
生物
分子生物学
材料科学
组合化学
癌症
基因
小RNA
遗传学
转移
膜
作者
Ke Kang,Yujia Zhang,Xiaoxi Zhou,Yue Yu,Nanhang Zhu,Jia Cheng,Qiangying Yi,Yao Wu
标识
DOI:10.1002/adhm.202202825
摘要
Abstract The capture of melanoma circulating tumor cells (melanoma CTCs, MelCTCs) is of great significance for the early diagnosis and personalized treatment of melanoma. The rarity and heterogeneity of MelCTCs have greatly limited the development of MelCTCs capture methods, especially those based on immune/aptamer‐affinity. Herein, an extracellular vesicles‐camouflaged strategy is designed to functionalize the magnetic nanoparticles (Fe 3 O 4 ) and to generate magnetic vesicles (Fe 3 O 4 @lip/ev) with excellent antifouling and active tumor cell targeting properties. Combined with the bioorthogonal click chemistry, the engineered magnetic vesicles with dibenzocyclooctyne can be widely used to target and separate all the metabolically labeled CTCs with varied phenotypes, organ origin, and even the biological species. The capture efficiency exceeded 80% with an extremely low detection limitation of ten cells. Most importantly, the strategy proposed can be directly applied to enrich MelCTCs from 0.5 mL blood samples of melanoma‐bearing mice, with a greatly minimized residue of white blood cells (only 21–568) while ignoring the fluctuations of MelCTC phenotype.
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