抗坏血酸
X射线光电子能谱
检出限
吸光度
催化作用
化学
扫描电子显微镜
核化学
吸附
透射电子显微镜
电子转移
分析化学(期刊)
材料科学
纳米技术
光化学
色谱法
物理化学
有机化学
化学工程
食品科学
工程类
复合材料
作者
Jin Lin Yang,Shiqi Cheng,Shangying Qin,Lan Huang,Yuanjin Xu,Yilin Wang
出处
期刊:RSC Advances
[The Royal Society of Chemistry]
日期:2023-01-01
卷期号:13 (15): 9918-9923
被引量:10
摘要
A CeO2-Co3O4 nanocomposite (NC) was prepared and characterized by scanning electron microscopy, transmission electron microscopy, X-ray photoelectron spectroscopy and X-ray diffraction. The obtained CeO2-Co3O4 NC displayed biomimicking oxidase-like activity, which can catalytically oxidize the 3, 3', 5, 5'-tetramethylbenzidine (TMB) substrate from colorless to the blue oxidized TMB (ox-TMB) product with a characteristic absorption peak at 652 nm. When ascorbic acid (AA) was present, ox-TMB would be reduced, resulting in a lighter blue and lower absorbance. On the basis of these facts, a simple colorimetric method for detection of AA was established with a linear relationship ranging from 1.0 to 500 μM and a detection limit of 0.25 μM. When this method was used to detect AA in human serum and commercially available vitamin C tablet samples, a good recovery of 92.0% to 109.0% was obtained. Besides, the catalytic oxidation mechanism was investigated, and the possible catalytic mechanism of CeO2-Co3O4 NC can be described as follows. TMB is adsorbed on the CeO2-Co3O4 NC surface and provides lone-pair electrons to the CeO2-Co3O4 NC, leading to an increase in electron density of the CeO2-Co3O4 NC. An increased electron density can improve the electron transfer rate between TMB and the oxygen absorbed on its surface to generate O2˙- and ˙O2, which further oxidize TMB.
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