细胞凋亡
软骨细胞
阿格里坎
污渍
小干扰RNA
衰老
化学
II型胶原
流式细胞术
基质金属蛋白酶
软骨
骨关节炎
炎症
阿达姆斯
细胞生物学
癌症研究
体外
生物
分子生物学
免疫学
转染
医学
金属蛋白酶
病理
生物化学
血栓反应素
基因
替代医学
解剖
关节软骨
作者
Wenxiang Cai,Haohuan Li,Wenyi Jin,Sixing Wei,Junwen Chen,Changheng Zhong,Yujian Zhong,Chang Tu,Hao Peng
标识
DOI:10.1016/j.intimp.2022.109336
摘要
Osteoarthritis (OA) is characterized by cartilage degeneration and inflammation. Procyanidin B2 (PCB2), a natural flavonoid compound, exhibits potential anti-inflammatory and anti-oxidative effects against several diseases. However, its curative effects on OA remain unclear.Herein, we explored the anti-arthritic effects of PCB2 on OA onset and progress and its potential mechanism.CCK-8 assays and EdU staining were used to assess the cytotoxic effects and cell proliferation activity of PCB2. Flow cytometry was used to detect apoptosis in chondrocytes. ELISA, qPCR, and western blotting, were applied to explore the expression of apoptosis and senescence-associated secretion phenotype (SASP) factors. The Nrf2/NF-κB signaling cascade was explored using immunofluorescence and western blotting. Additionally, we silenced the Nrf2 gene using siRNAs to verify its function in PCB2 regulation of senescence and apoptosis phenotypes. Safranin O-Fast Green (SO) and immunohistochemical staining were used to explore the effects of PCB2 on OA model rats.PCB2 dampened interleukin (IL)-1β-triggered expression of SASP factors in vitro. Additionally, PCB2 diminished IL-1β-triggered destruction of the extracellular matrix (ECM) via downregulating the expression of MMPs, while upregulating the expression of collagen II and aggrecan. In addition, PCB2 treatment reduced IL-1β-induced apoptosis of chondrocytes. Mechanistically, PCB2 could attenuated chondrocyte senescence in vitro via the Nrf2/NF-κB pathway. Moreover, PCB2 exhibited anti-apoptotic properties via the Nrf2/BAX/Bcl-2 pathway. PCB2 alleviated knee cartilage degeneration in an OA rat model.Our results suggest that PCB2 may be used as a therapeutic agent for OA.
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