活性氧
拟南芥
拟南芥
植物种类
生物
离体
植物
生物物理学
化学
细胞生物学
体外
生物化学
突变体
基因
作者
Ronald J. Myers,Sara I. Zandalinas,Ron Mittler
出处
期刊:Methods in molecular biology
日期:2023-01-01
卷期号:: 387-401
标识
DOI:10.1007/978-1-0716-3044-0_21
摘要
Reactive Oxygen Species (ROS)Reactive oxygen species (ROS) wavesWaves serve as key systemicSystemic signals within plants. Following the initial sensation of a stress, auto-propagation of ROSReactive oxygen species (ROS) (the ROS waveROS wave) begins and rapidly spreads to distant, systemicSystemic tissues of the plant and invokes important physiological responses. Highly sensitive methods capable of imaging this systemicSystemic signal at the whole-plantWhole-plant level have long been desired for the study of ROS signalingROS signaling. Here, we describe a straightforward and highly sensitive method for the detection and quantification of ROSReactive oxygen species (ROS) in planta at the whole-plantWhole-plant level in Arabidopsis thaliana with the In Vivo Imaging System (IVIS)In Vivo Imaging System (IVIS) Lumina S5 imaging platform and the fluorescent probeFluorescent probe 2′,7′-dichlorofluorescin diacetate2’,7’-dichlorofluorescin diacetate (HDCFDA) (H2DCFDA). This method can be used for high-throughput screening of the ROS WaveROS wave within Arabidopsis plants, with up to 16 plants capable of being imaged approximately every half hour.
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