217P Circulating microRNA21 and microRNA126: Diagnostic, prognostic value and multivariate analysis in non-small cell lung cancer

Background: One of the most recent approaches of tumor molecular characterization is mainly based on microRNA expression profile. No single marker is sufficiently accurate for clinical use and multiple biomarker panels are developed for three main purposes tumor subtype classification, early detection and prediction of tumor responses to treatment and prognosis of patients. Micro- 21 and Micro-126 have received special attention because of their relationship with many cancer sites we aimed to study their diagnostic and prognostic utility in lung cancer patients. Methods: 100 subjects classified into two groups: group 1 comprised 60 Lung cancer patients, and group II comprised 40 age- and sex-matched volunteers, Real-time PCR of micro RNA 21 and 126 were done and studied in control and patients to detect diagnostic utility and correlated with all disease clinicopathological data and patients survival. Results: Higher miR-21 and lower miR-126 levels were found in lung patients than in controls. The sensitivity of CEA and miR-21 and miR-126 (78.3%, 96.7%, 90%) at cutoff points (7.5, 2.35, 2.175) respectively to distinguish NSCLC patients from controls. On combining both microRNA21 & microRNA 126 an improvement of sensitivity to 97% was noted. For patients, miR-21 increased significantly with metastatic stage and highest grade GIII. Regarding survival there was significantly longer overall survival among patients with more early stages and lower grades GI &II and with low Micro- 21 and high micro-126. On Cox regression analysis for independent prognostic factors of survival; micro-126 and presence of metstases were the independent factor for survival with hazard ratio 0.26 (95% CI 0.06–1.09) 3.64 (95% CI 1.22–16.5) respectively. Conclusions: CirculatorymiR-21 and miR-126 may play significant role in diagnosis and prognosis in NSCLC patients. Legal entity responsible for the study: Menoufia University. Funding: Has not received any funding. Disclosure: All authors have declared no conflicts of interest.