磺胺二甲嘧啶
检出限
化学
电极
生物传感器
电化学
纳米复合材料
胶体金
线性范围
石墨烯
氧化还原
纳米技术
纳米颗粒
组合化学
核化学
色谱法
材料科学
无机化学
物理化学
作者
Aiping Wang,Chenyang Zhang,Xiaojuan You,Jingming Zhou,Yumei Chen,Chao Liang,Dong-Dong Ma,Dan Liu,Gaiping Zhang
标识
DOI:10.1002/slct.202102260
摘要
Abstract Sulfadimidine (SM2) is a kind of synthetic sulfadiazine antibiotics. Excessive use of SM2 in animal breeding can lead to animal‐derived food residues, which will bring potential risks to human health. Herein, a sensitive label‐free electrochemical immunosensor using silver nanoparticles (AgNPs) as redox probe was developed for the specific detection of SM2. Firstly, the Ag−GO nanocomposites were prepared by in‐situ reduction of AgNPs on graphene oxide (GO) surface with glucose. Gold nanoparticles (AuNPs) were reduced on the surface of Ag−GO nanocomposites by permanent electrodeposition. Staphylococcal protein A (SPA) was dripped onto the AuNPs modified electrode to absorb antibody (anti‐SM2). Herein, GO provided a large surface area for assembling a large number of AgNPs with redox activity. AuNPs were used to immobilize SPA and amplify the electrochemical signal. In addition, SPA with antibody targeting function was introduced to improve the efficiency of immune response. Under optimal conditions, the label‐free immunosensor showed a linear range of 63 pg/mL to 20 ng/mL with a limit of detection (LOD) of 3 pg/mL (S/N=3). Finally, the developed method showed excellent performance in practical application, which provided a promising possibility for the detection of other veterinary drug residues in animal derived food.
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