卡普萨平
胡椒碱
化学
生物物理学
三叉神经节
TRPV1型
辣椒素
药理学
神经科学
瞬时受体电位通道
生物化学
受体
医学
生物
有机化学
感觉系统
作者
Melissa E. Martenson,Joseph H Arguelles,Thomas Baumann
出处
期刊:Brain Research
[Elsevier]
日期:1997-06-01
卷期号:761 (1): 71-76
被引量:14
标识
DOI:10.1016/s0006-8993(97)00305-3
摘要
Both trigeminal and spinal ganglion neurons show a strong potentiation of responses to the irritant capsaicin in an acidic environment. The present study revealed that there is also a strong interaction between protons and piperine, another vanilloid irritant. We studied the mechanism of the interaction between protons and piperine. Whole-cell patch clamp recordings were performed on cultured adult rat trigeminal ganglion (TG) neurons voltage-clamped near their resting membrane potential (−60 mV). Piperine (10 μM) caused a sustained net inward current associated with either an increase or decrease in membrane conductance. When protons and piperine were co-applied, the membrane currents evoked in piperine-sensitive TG neurons far exceeded the algebraic sum of the responses to the two stimuli applied in isolation. Capsazepine blocked the response of TG neurons to piperine at both physiological and acidic pH. In the presence of capsazepine, responses to the mixture of piperine and protons resembled the response to the low pH stimulus applied alone. Capsazepine had no effect on the sustained proton-induced current. These findings suggest that protons enhance the piperine current by altering the vanilloid receptor/channel complex or increasing the length constant of the space clamp.
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