DNA甲基化
染色质免疫沉淀
甲基化DNA免疫沉淀
甲基化
生物
人类基因组
RNA导向的DNA甲基化
表观遗传学
发起人
照明菌甲基化试验
计算生物学
DNA
体育锻炼的表观遗传学
基因
染色质
遗传学
基因组
基因表达
作者
Kelsie L. Thu,Larissa A. Pikor,Jennifer Y. Kennett,Carlos E. Alvarez,Wan L. Lam
摘要
Abstract DNA methylation regulates gene expression primarily through modification of chromatin structure. Global methylation studies have revealed biologically relevant patterns of DNA methylation in the human genome affecting sequences such as gene promoters, gene bodies, and repetitive elements. Disruption of normal methylation patterns and subsequent gene expression changes have been observed in several diseases especially in human cancers. Immunoprecipitation (IP)‐based methods to evaluate methylation status of DNA have been instrumental in such genome‐wide methylation studies. This review describes techniques commonly used to identify and quantify methylated DNA with emphasis on IP based platforms. In an effort to consolidate the wealth of information and highlight critical aspects of methylated DNA analysis, sample considerations, experimental and bioinformatic approaches for analyzing genome‐wide methylation profiles, and the benefit of integrating DNA methylation data with complementary dimensions of genomic data are discussed. J. Cell. Physiol. 222: 522–531, 2010. © 2009 Wiley‐Liss, Inc.
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