IR-780-Albumin-Based Nanocarriers Promote Tumor Regression Not Only from Phototherapy but Also by a Nonirradiation Mechanism

体内分布 光热治疗 纳米载体 牛血清白蛋白 化学 医学 生物物理学 癌症研究 体内 阿霉素 纳米颗粒 体外 材料科学 纳米技术 生物化学 生物 化疗 内科学 生物技术
作者
Gustavo Capistrano,Ailton A. Sousa-Junior,Roosevelt Alves da Silva,Francyelli Mello-Andrade,Emílio R. Cintra,Sônia F. O. Santos,Allancer Nunes,Raisa Melo Lima,Nícholas Zufelato,André Schneider de Oliveira,Maristela Pereira,Carlos H. Castro,Eliana Martins Lima,Cléver Gomes Cardoso,Elisângela de Paula Silveira-Lacerda,Sebastião Antônio Mendanha,Andris F. Bakuzis
出处
期刊:ACS Biomaterials Science & Engineering [American Chemical Society]
卷期号:6 (8): 4523-4538 被引量:22
标识
DOI:10.1021/acsbiomaterials.0c00164
摘要

IR-780 iodide is a fluorescent dye with optical properties in the near-infrared region that has applications in tumor detection and photothermal/photodynamic therapy. This multifunctional effect led to the development of theranostic nanoparticles with both IR-780 and chemotherapeutic drugs such as docetaxel, doxorubicin, and lonidamine. In this work, we developed two albumin-based nanoparticles containing near-infrared IR-780 iodide multifunctional dyes, one of them possessing a magnetic core. Molecular docking with AutoDock Vina studies showed that IR-780 binds to bovine serum albumin (BSA) with greater stability at a higher temperature, allowing the protein binding pocket to better fit this dye. The theoretical analysis corroborates the experimental protocols, where an enhancement of IR-780 was found coupled to BSA at 60 °C, even 30 days after preparation, in comparison to 30 °C. In vitro assays monitoring the viability of Ehrlich ascites carcinoma cells revealed the importance of the inorganic magnetic core on the nanocarrier photothermal–cytotoxic effect. Fluorescence molecular tomography measurements of Ehrlich tumor-bearing Swiss mice revealed the biodistribution of the nanocarriers, with marked accumulation in the tumor tissue (≈3% ID). The histopathological analysis demonstrated strong increase in tumoral necrosis areas after 24 and 72 h after treatment, indicating tumor regression. Tumor regression analysis of nonirradiated animals indicate a IR-780 dose-dependent antitumoral effect with survival rates higher than 70% (animals monitored up to 600 days). Furthermore, an in vivo photothermal therapy procedure was performed and tumor regression was also verified. These results show a novel insight for the biomedical application of IR-780-albumin-based nanocarriers, namely cancer therapy, not only by photoinduced therapy but also by a nonirradiation mechanism. Safety studies (acute oral toxicity, cardiovascular evaluation, and histopathological analysis) suggest potential for clinical translation.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
谨慎鹏涛完成签到 ,获得积分10
11秒前
Jessie完成签到 ,获得积分10
15秒前
18秒前
丽丽完成签到,获得积分10
31秒前
33秒前
坐宝马吃地瓜完成签到 ,获得积分10
50秒前
52秒前
烟花应助拾陆采纳,获得10
1分钟前
伶俐绿柏完成签到 ,获得积分10
1分钟前
研友_VZG7GZ应助Ilyas0525采纳,获得10
1分钟前
1分钟前
禾页完成签到 ,获得积分10
1分钟前
CorisKen应助端庄的萝采纳,获得20
1分钟前
打打应助Ilyas0525采纳,获得10
1分钟前
晁子枫完成签到 ,获得积分10
1分钟前
张希伦完成签到 ,获得积分10
1分钟前
Emma发布了新的文献求助80
1分钟前
1分钟前
科目三应助Ilyas0525采纳,获得10
1分钟前
DeepLearning发布了新的文献求助10
1分钟前
Jasmineyfz完成签到 ,获得积分10
1分钟前
1分钟前
Ilyas0525完成签到,获得积分10
2分钟前
Emma完成签到,获得积分10
2分钟前
小木林完成签到 ,获得积分10
2分钟前
朴素羊完成签到 ,获得积分10
2分钟前
辛勤的喉完成签到 ,获得积分10
2分钟前
yunt完成签到 ,获得积分10
2分钟前
林利芳完成签到 ,获得积分0
2分钟前
神勇的翠丝完成签到,获得积分10
2分钟前
xiangwang完成签到 ,获得积分10
2分钟前
Joan_89完成签到,获得积分10
2分钟前
juliar完成签到 ,获得积分10
2分钟前
Akim应助DeepLearning采纳,获得10
2分钟前
迈克老狼完成签到 ,获得积分10
2分钟前
Kelevator完成签到,获得积分10
2分钟前
DeepLearning完成签到,获得积分20
2分钟前
2分钟前
DeepLearning发布了新的文献求助10
2分钟前
SOL完成签到 ,获得积分10
3分钟前
高分求助中
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3990836
求助须知:如何正确求助?哪些是违规求助? 3532241
关于积分的说明 11256631
捐赠科研通 3271100
什么是DOI,文献DOI怎么找? 1805313
邀请新用户注册赠送积分活动 882302
科研通“疑难数据库(出版商)”最低求助积分说明 809236