Single-Cell Determination of Cardiac Microtissue Structure and Function Using Light Sheet Microscopy

多细胞生物 电池类型 细胞 心肌细胞 心肌细胞 单细胞分析 人口 细胞生物学 生物 生物物理学 医学 生物化学 环境卫生
作者
Diwakar Turaga,Oriane B. Matthys,Tracy A. Hookway,David A. Joy,Meredith Calvert,Todd C. McDevitt
出处
期刊:Tissue Engineering Part C-methods [Mary Ann Liebert, Inc.]
卷期号:26 (4): 207-215 被引量:10
标识
DOI:10.1089/ten.tec.2020.0020
摘要

Native cardiac tissue is composed of heterogeneous cell populations that work cooperatively for proper tissue function; thus, engineered tissue models have moved toward incorporating multiple cardiac cell types in an effort to recapitulate native multicellular composition and organization. Cardiac tissue models composed of stem cell-derived cardiomyocytes (CMs) require inclusion of non-myocytes to promote stable tissue formation, yet the specific contributions of the supporting non-myocyte population on the parenchymal CMs and cardiac microtissues have to be fully dissected. This gap can be partly attributed to limitations in technologies able to accurately study the individual cellular structure and function that comprise intact three-dimensional (3D) tissues. The ability to interrogate the cell–cell interactions in 3D tissue constructs has been restricted by conventional optical imaging techniques that fail to adequately penetrate multicellular microtissues with sufficient spatial resolution. Light sheet fluorescence microscopy (LSFM) overcomes these constraints to enable single-cell resolution structural and functional imaging of intact cardiac microtissues. Multicellular spatial distribution analysis of heterotypic cardiac cell populations revealed that CMs and cardiac fibroblasts were randomly distributed throughout 3D microtissues. Furthermore, calcium imaging of live cardiac microtissues enabled single-cell detection of CM calcium activity, which showed that functional heterogeneity correlated with spatial location within the tissues. This study demonstrates that LSFM can be utilized to determine single-cell spatial and functional interactions of multiple cell types within intact 3D engineered microtissues, thereby facilitating the determination of structure–function relationships at both tissue-level and single-cell resolution. The ability to achieve single-cell resolution by advanced three-dimensional light imaging techniques enables exquisite new investigation of multicellular analyses in native and engineered tissues. In this study, light sheet fluorescence microscopy was used to define structure–function relationships of distinct cell types in engineered cardiac microtissues by determining heterotypic cell distributions and interactions throughout the tissues as well as by assessing regional differences in calcium handing functional properties at the individual cardiomyocyte level.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
3秒前
4秒前
pp发布了新的文献求助10
5秒前
顾矜应助小李博士采纳,获得10
5秒前
8秒前
nichen发布了新的文献求助10
8秒前
dustomb发布了新的文献求助10
10秒前
醉熏的天与应助pp采纳,获得10
12秒前
CodeCraft应助ecnuZhao采纳,获得10
12秒前
13秒前
冰激凌UP发布了新的文献求助10
13秒前
逸兴遄飞完成签到,获得积分20
14秒前
14秒前
cyn完成签到,获得积分10
17秒前
jackwang完成签到,获得积分10
19秒前
竹筏过海应助雪山飞龙采纳,获得30
19秒前
19秒前
georgia_qiao完成签到,获得积分10
20秒前
kuoping完成签到,获得积分0
20秒前
逸兴遄飞发布了新的文献求助30
20秒前
21秒前
21秒前
22秒前
25秒前
小李博士发布了新的文献求助10
26秒前
26秒前
26秒前
27秒前
ding应助呵呵采纳,获得10
29秒前
无瑕玉完成签到,获得积分10
30秒前
背后孤晴完成签到,获得积分10
31秒前
ttkx完成签到,获得积分10
31秒前
乔垣结衣应助雪山飞龙采纳,获得10
31秒前
紫心发布了新的文献求助10
31秒前
Stanford发布了新的文献求助10
31秒前
我是老大应助123采纳,获得10
33秒前
科研通AI2S应助小李博士采纳,获得10
33秒前
ecnuZhao发布了新的文献求助10
33秒前
simctz完成签到,获得积分10
33秒前
33秒前
高分求助中
Ophthalmic Equipment Market by Devices(surgical: vitreorentinal,IOLs,OVDs,contact lens,RGP lens,backflush,diagnostic&monitoring:OCT,actorefractor,keratometer,tonometer,ophthalmoscpe,OVD), End User,Buying Criteria-Global Forecast to2029 2000
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3962850
求助须知:如何正确求助?哪些是违规求助? 3508775
关于积分的说明 11142938
捐赠科研通 3241643
什么是DOI,文献DOI怎么找? 1791625
邀请新用户注册赠送积分活动 872998
科研通“疑难数据库(出版商)”最低求助积分说明 803571