黑腹果蝇
外围设备
体内
轴突
周围神经病变
生物
感觉系统
果蝇属(亚属)
神经科学
医学
病理
生物技术
内科学
遗传学
基因
内分泌学
糖尿病
作者
Martha R.C. Bhattacharya
出处
期刊:Methods in molecular biology
日期:2020-01-01
卷期号:: 301-310
被引量:1
标识
DOI:10.1007/978-1-0716-0585-1_22
摘要
Peripheral neuropathies are one of the largest categories of neurodegenerative diseases. To investigate their mechanisms, many in vitro and in vivo models can be employed. Here we present a protocol for the induction of chemotherapy-induced peripheral neuropathy (CIPN) in the Drosophila melanogaster (fruit fly) model system. Using a clinically relevant degeneration initiator, paclitaxel (taxol), it is possible to model many aspects of axon and dendrite degeneration while in a genetically tractable, in vivo system. In this protocol, we feed larval stage Drosophila neurotoxic chemotherapy drugs during the duration of larval development, followed by dissection and imaging of genetically labeled sensory axons and dendrites. Both axons and dendrites degenerate with taxol exposure. Our protocol should facilitate the adoption and expansion of this model to include other neurotoxic compounds.
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