Novel perspective into the interaction behavior study of the cyanidin with human serum albumin-holo transferrin complex: Spectroscopic, calorimetric and molecular modeling approaches

化学 等温滴定量热法 人血清白蛋白 圆二色性 结合常数 氰化物 荧光 猝灭(荧光) 三元络合物 分析化学(期刊) 结晶学 色谱法 物理化学 结合位点 花青素 生物化学 物理 量子力学 食品科学
作者
Sattar Khashkhashi-Moghadam,Sara Ezazi-Toroghi,Melika Kamkar-Vatanparast,Parisa Jouyaeian,Parisa Mokaberi,Helya Yazdyani,Zeinab Amiri‐Tehranizadeh,Mohammad Reza Saberi,Jamshidkhan Chamani
出处
期刊:Journal of Molecular Liquids [Elsevier BV]
卷期号:356: 119042-119042 被引量:110
标识
DOI:10.1016/j.molliq.2022.119042
摘要

We examined the inducement of an interaction between two carrier proteins, human serum albumin (HSA) and human holo transferrin (HTF) within the presence of cyanidinin the form of binary and ternary systems, which was conducted by different spectroscopic, isothermal titration calorimetric (ITC), and molecular dynamics simulation techniques. The results of fluorescence spectroscopy verified the occurrence of this interaction by displaying the regular quenching of emission spectra of proteins as single and complex forms by cyanidin. Moreover, fluorescence emission suggested that, HSA-cyanidin, HTF-cyanidin and (HSA-HTF) cyanidin complexes follows an static mechanism with the binding constants of 8.81 × 104, 3.40 × 104, and 7.10 × 104 M−1, respectively, which were strongly reflected to moderate the affinity of cyanidin to both single and complex forms of proteins. The measured thermodynamic parameters obtained by ITC, determined the necessity of electrostatic forces for binding of cyanidin to HSA and HTF. Besides, obtaining a negative ΔG0 is indicative of spontaneous mode of binding process in both conditions. The different values of thermodynamic parameters of cyanidin binding to the forms of HSA, HTF and HSA-HTF complex clearly proved the similarity in the type of interaction forces between cyanidin and proteins as single and complex forms with various values. The observed changes in synchronous fluorescence results were related to the micro-environment properties of Tyr and Trp residues. The induced alternations in the secondary construction of both proteins were confirmed through the outcomes of quantitative analysis performed by circular dichroism spectroscopy (CD) techniques. FRET theory was exerted to calculate, the existing distance across the donor and acceptor, which were reported to be 1.85 nm, 1.97 nm, and 1.91 nm for the cases of HSA-cyanidin, HTF-cyanidin and (HSA-HTF) cyanidin complexes, respectively. Molecular displacement and protein–ligand docking simulations confirmed the binding of cyanidin to HSA and HTF through the binary and ternary systems.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
孤鲸游完成签到,获得积分10
1秒前
1秒前
1秒前
鄙视注册完成签到,获得积分10
1秒前
1秒前
故乡风话完成签到,获得积分20
2秒前
2秒前
Eleanor给Eleanor的求助进行了留言
3秒前
晴岚风树完成签到,获得积分10
3秒前
4秒前
李健应助11号迪西馅饼采纳,获得10
4秒前
量子星尘发布了新的文献求助30
4秒前
4秒前
4秒前
4秒前
5秒前
5秒前
莉莉发布了新的文献求助10
5秒前
hss完成签到 ,获得积分10
5秒前
5秒前
顾矜应助xuan采纳,获得10
5秒前
junfeiwang发布了新的文献求助10
6秒前
6秒前
6秒前
芒果完成签到,获得积分10
6秒前
6秒前
kk应助微醺小王采纳,获得10
6秒前
科研科完成签到,获得积分10
6秒前
7秒前
Archer发布了新的文献求助10
7秒前
7秒前
活泼身影完成签到,获得积分10
8秒前
wos发布了新的文献求助10
8秒前
8秒前
9秒前
zho发布了新的文献求助10
9秒前
9秒前
9秒前
10秒前
10秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Picture Books with Same-sex Parented Families: Unintentional Censorship 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3969383
求助须知:如何正确求助?哪些是违规求助? 3514211
关于积分的说明 11172730
捐赠科研通 3249476
什么是DOI,文献DOI怎么找? 1794909
邀请新用户注册赠送积分活动 875441
科研通“疑难数据库(出版商)”最低求助积分说明 804827