Development of a Biomimetic Hydrogel Based on Predifferentiated Mesenchymal Stem‐Cell‐Derived ECM for Cartilage Tissue Engineering

去细胞化 间充质干细胞 自愈水凝胶 细胞外基质 软骨 软骨发生 组织工程 透明软骨 生物医学工程 再生(生物学) 细胞生物学 生物材料 再生医学 化学 干细胞 基质(化学分析) 材料科学 解剖 关节软骨 病理 生物 骨关节炎 医学 复合材料 有机化学 替代医学
作者
Cristina Antich,Gema Jiménez,Juan de Vicente,Elena López‐Ruiz,Carlos Chocarro‐Wrona,Carmen Griñán‐Lisón,Esmeralda Carrillo,Elvira Montañez-Heredia,Juan Antonio Marchal
出处
期刊:Advanced Healthcare Materials [Wiley]
卷期号:10 (8) 被引量:39
标识
DOI:10.1002/adhm.202001847
摘要

Abstract The use of decellularized extracellular matrix (dECM) as a biomaterial has been an important step forward for the development of functional tissue constructs. In addition to tissues and organs, cell cultures are gaining a lot of attention as an alternative source of dECM. In this work, a novel biomimetic hydrogel is developed based on dECM obtained from mesenchymal stem cells (mdECM) for cartilage tissue engineering. To this end, cells are seeded under specific culture conditions to generate an early chondrogenic extracellular matrix (ECM) providing cues and elements necessary for cartilage development. The composition is determined by quantitative, histological, and mass spectrometry techniques. Moreover, the decellularization process is evaluated by measuring the DNA content and compositional analyses, and the hydrogel is formulated at different concentrations (3% and 6% w/v). Results show that mdECM derived hydrogels possess excellent biocompatibility and suitable physicochemical and mechanical properties for their injectability. Furthermore, it is evidenced that this hydrogel is able to induce chondrogenesis of mesenchymal stem cells (MSCs) without supplemental factors and, furthermore, to form hyaline cartilage‐like tissue after in vivo implantation. These findings demonstrate for the first time the potential of this hydrogel based on mdECM for applications in cartilage repair and regeneration.
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