聚乙二醇
PEG比率
色谱法
化学
试剂
牛血清白蛋白
大小排阻色谱法
结合
氯仿
水溶液
生物化学
有机化学
数学分析
数学
财务
经济
酶
作者
Alo Nag,Gargi Mitra,Prahlad C. Ghosh
标识
DOI:10.1006/abio.1996.0233
摘要
A colorimetric method for quantitative assay of polyethylene glycol (PEG) described here is based on partitioning of a chromophore present in ammonium ferrothiocyanate reagent from an aqueous to a chloroform phase in the presence of PEG. The method is simple, reproducible, and can detect PEG in amounts as low as 5 μg. It gives a linear response over a range of 5–100 μg. The absence of any interference by proteins makes the assay equally suitable for the estimation of PEG in PEG–protein conjugates. The method was employed to monitor the separation profile of a mixture of free and PEG-5000 coupled to bovine serum albumin during purification through a gel filtration column. In this report we have also demonstrated for the first time an assay method which permits a critical evaluation of pharmacokinetic properties of any PEG–protein conjugate underin vivoconditions.
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