Application of an Endophyte Enterobacter sp. TMX13 to Reduce Thiamethoxam Residues and Stress in Chinese Cabbage (Brassica chinensis L)

A strain of thiamethoxam-degrading endophyte, named TMX13, was isolated from roots of mulberry (Morus alba L.) and was identified as Enterobacter sp. Inoculating Chinese cabbage (Brassica chinensis L) with strain TMX13-gfp (gfp-labeled TMX13) could significantly reduce thiamethoxam residues in the aboveground part (edible portion) of the vegetable. The theoretical daily intake (TDI) of thiamethoxam via consumption of TMX13-gfp inoculated Chinese cabbage was 0.17 μg/kg body weight per day, far less than the prescribed acceptable daily intake (ADI) for this pesticide. TMX13-gfp colonization could increase the leaf chlorophyll content and plant biomass and promote the development of plant roots. Compared with the uninoculated treatment, the contents of malondialdehyde (MDA) and hydrogen peroxide (H2O2) and the activity of superoxide dismutase (SOD) in leaves of the TMX13-gfp inoculated plants decreased by 18.4%–60.2%, suggesting that TMX13-gfp colonization could alleviate the oxidative stress induced by thiamethoxam exposure. The total amounts of organic acids and amino acids in root exudates from the TMX13-gfp inoculated Chinese cabbage decreased by 9.2% and 85.2%, respectively. Results of this study lead to the conclusion that the isolated endophyte Enterobacter sp. TMX13 could reduce thiamethoxam residues in edible vegetables, promote plant growth, and alleviate the phytotoxic effects induced by thiamethoxam exposure.