氧化应激
碳水化合物
半乳糖
谷胱甘肽
抗氧化剂
化学
过氧化氢酶
单糖
超氧化物歧化酶
糖苷键
果糖
生物化学
酶
作者
Junyuan Huang,Yanlan Chen,Yi Su,Wanqing Yuan,Dong Peng,Ziwen Guan,Jianping Chen,Pan Li,Bing Du
标识
DOI:10.1016/j.ijbiomac.2024.129760
摘要
The specific structure of Polygonatum kingianum Coll. et Hemsl polysaccharide (PKP) has been rarely reported. In this study, an inulin-type fructan PKP-1, was extracted and purified from Polygonatum kingianum Coll. et Hemsl, and its structural characteristics and antioxidants activity were evaluated. The molecular weights of PKP-1 was determined to be 4.802 kDa. Monosaccharide composition analysis evidenced that PKP-1 was composed of galactose, glucose and fructose in a molar ratio of 0.8 %:7.2 %:92.0 %. Glycosidic linkage and Nuclear Magnetic Resonance (NMR) analysis revealed that PKP-1 exhibited a primary sugar residue linkage of →1-β-d-Fruf-2→2,6-β-d-Fruf-1→, where β-d-Fruf-2→ acts as the side chain and links to the C-6 position of →2,6-β-d-Fruf-1→. In vitro antioxidant activity assays demonstrated that PKP-1 enhanced the mitigation of hepatic oxidative stress in HepG2 cells induced by free fatty acids. This effect was marked by increased enzymatic activities of superoxidase dismutase (SOD) and catalase (CAT), along with elevated glutathione (GSH) levels. These findings indicate that PKP-1 could be used as a potential natural antioxidant.
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