Locking-DNA network regulated CRISPR-Cas12a fluorescent aptasensor based on hollow flower-like magnetic MoS2 microspheres for sensitive detection of sulfadimethoxine

费斯特共振能量转移 荧光 清脆的 适体 多路复用 检出限 猝灭(荧光) 化学 纳米技术 材料科学 色谱法 生物 物理 基因 分子生物学 生物化学 量子力学 生物信息学
作者
Yan Lv,Yuhan Sun,Imran Mahmood Khan,Qiaofeng Li,You Zhou,Lin Yue,Yin Zhang,Zhouping Wang
出处
期刊:Chemical Engineering Journal [Elsevier BV]
卷期号:459: 141463-141463 被引量:18
标识
DOI:10.1016/j.cej.2023.141463
摘要

Sulfadimethoxine (SDM) poses a potential threat to the food safety and human health due to its overuse in aquatic or animal husbandry. Therefore, a rapid and sensitive identification method is urgently needed to monitor SDM in food and the environment. Herein, a “turn-on” fluorescence aptasensor based on entropy-driven catalysis (EDC) and CRISPR-Cas12a was proposed for the sensitive determination of SDM. Particularly, to overcome the barriers of coupling the EDC circuit to CRISPR-Cas12a, a novel EDC circuit with a Y-shaped substrate scaffold was designed to lock the activation sequence of CRISPR-Cas12a. Multifunctional hollow flower-like microsphere H-Fe3O4@MoS2 was synthesized as a fluorescence resonance energy transfer (FRET) energy acceptor for ssDNA-FAM to quenching its fluorescence. In the presence of the SDM, activated Cas12a-crRNA-A rapidly cleaved the ssDNA-FAM adsorbed on the MoS2 nanosheet to restore the fluorescence signal in the reporter system, which could be utilized to determine the concentration of SDM. Due to the prominent quenching properties and rapid magnetic separation of H-Fe3O4@MoS2 along with the double signal amplification of locking-EDC-CRISPR-Cas12a, the developed aptasensor exhibited the appealing analytical performance with a log-linear range of 0.005–100 ng mL−1 and a limit of detection of 2.86 pg mL−1. Furthermore, the practicability and credibility of the locking-EDC-Cas12a sensoring strategy were validated by spiked recovery experiments of SDM in real samples. Hence, the current strategy provides a promising candidate analytical tool for food and environmental safety.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
高贵梦露发布了新的文献求助10
1秒前
3秒前
4秒前
GL发布了新的文献求助10
4秒前
乐乐应助如意枫叶采纳,获得10
4秒前
5秒前
史念薇发布了新的文献求助10
5秒前
xixi完成签到 ,获得积分10
5秒前
7秒前
青野发布了新的文献求助10
9秒前
12秒前
13秒前
高贵梦露完成签到,获得积分10
13秒前
15秒前
17秒前
量子星尘发布了新的文献求助10
18秒前
如意枫叶发布了新的文献求助10
19秒前
19秒前
20秒前
20秒前
22秒前
23秒前
24秒前
赘婿应助GL采纳,获得10
24秒前
25秒前
25秒前
Archer宇完成签到,获得积分10
25秒前
狸花小喵发布了新的文献求助10
27秒前
科研废物完成签到 ,获得积分10
27秒前
28秒前
29秒前
30秒前
31秒前
33秒前
34秒前
潇湘雪月发布了新的文献求助10
35秒前
35秒前
36秒前
ding应助咩咩羊采纳,获得10
36秒前
37秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3989263
求助须知:如何正确求助?哪些是违规求助? 3531418
关于积分的说明 11253814
捐赠科研通 3270066
什么是DOI,文献DOI怎么找? 1804884
邀请新用户注册赠送积分活动 882084
科研通“疑难数据库(出版商)”最低求助积分说明 809136