光电流
适体
材料科学
检出限
电极
氧化铟锡
表面等离子共振
可见光谱
纳米技术
光电子学
纳米颗粒
化学
薄膜
色谱法
遗传学
物理化学
生物
作者
Rumeng Song,Ying Li,Chi Zhang,Gaiping Li,Lijie Zou
标识
DOI:10.1016/j.microc.2023.109688
摘要
A signal off type photoelectrochemical (PEC) aptasensor was constructed for the sensitive detection of chloramphenicol based on CuO nanosheets (CuO NSs) and gold nanoflowers (Au NFs) modified indium tin oxide (ITO) electrode. CuO NSs was p-type photoactive semiconductor materials, which had narrow band gap and high absorption rate of visible light. After combining with Au NFs, the composite CuO NSs@Au NFs produced a strong cathodic photocurrent for the excellent electron conductivity, large specific surface area and Surface Plasmon Resonance effect (SPR) of Au NFs. At the same time, Au NFs provided a binding site for aptamer, which greatly improved the sensitivity of the constructed sensing platform. After the target chloramphenicol (CAP) was specifically captured by the aptamers on the electrode, the formed bioconjugates seriously hindered the transfer of electrons and the absorption of light and caused a reduced photocurrent. For the detection of CAP, this signal off type PEC aptasensor had a linear range of 5 pM-300 nM and a limit of detection of 49.9 fM (S/N = 3) with good stability, selectivity and reproducibility. Meanwhile, the method had satisfactory recovery results in the detection of CAP in real-life samples such as milk, tap water, honey and chloramphenicol eye drops.
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