脂质代谢
褪黑素
线粒体
新陈代谢
线粒体毒性
毒性
生物
脂肪酸代谢
功能(生物学)
内分泌学
内科学
细胞生物学
化学
生物化学
医学
作者
Tian-Ning Yang,Yuxiang Wang,Ping-An Jian,Xiangyu Ma,Yi-Fei Ren,Ningning Huang,Xue‐Nan Li,Jin‐Long Li
标识
DOI:10.1021/acs.jafc.4c07006
摘要
Atrazine (ATZ), a widely used herbicide, disrupts mitochondrial function and lipid metabolism in the liver. Melatonin (MLT), a naturally synthesized hormone, combats mitochondrial dysfunction and alleviates lipid toxicity. However, the mechanisms behind ATZ-induced lipid metabolism toxicity and the protective effects of MLT remain unexplored. Mice were randomly assigned to four groups: control (Con), 5 mg/kg MLT, 170 mg/kg ATZ, and a cotreatment group receiving 170 mg/kg ATZ with 5 mg/kg MLT (ATZ+MLT). Additionally, we analyzed the effects of MLT and Rab8a on mRNA and proteins related to mitochondrial function and lipid metabolism disrupted by ATZ in AML12 cells. In conclusion, ATZ induced mitochondrial stress and disrupted fatty acid metabolism in mouse hepatocytes and AML12 cells. Exogenous MLT restores Rab8a levels, regulating fatty acid utilization in mitochondria and mitochondrial function. Notably, targeting Rab8a does not significantly affect mitochondrial function but prevents ATZ-induced lipid metabolism disorders in hepatocytes.
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