单核细胞增生李斯特菌
化学发光
沙门氏菌
化学
微生物学
李斯特菌
生物
色谱法
细菌
遗传学
作者
Michal E. Roth‐Konforti,Ori Green,Mario Hupfeld,Lars Fieseler,Nadine Heinrich,Julian Ihssen,Raffael Vorberg,Lukas Y. Wick,Urs Spitz,Doron Shabat
标识
DOI:10.1002/anie.201904719
摘要
Abstract Detection of Salmonella and L. monocytogenes in food samples by current diagnostic methods requires relatively long time to results (2–6 days). Furthermore, the ability to perform environmental monitoring at the factory site for these pathogens is limited due to the need for laboratory facilities. Herein, we report new chemiluminescence probes for the ultrasensitive direct detection of viable pathogenic bacteria. The probes are composed of a bright phenoxy‐dioxetane luminophore masked by triggering group, which is activated by a specific bacterial enzyme, and could detect their corresponding bacteria with an LOD value of about 600‐fold lower than that of fluorescent probes. Moreover, we were able to detect a minimum of 10 Salmonella cells within 6 h incubation. The assay allows for bacterial enrichment and detection in one test tube without further sample preparation. We anticipate that this design strategy will be used to prepare analogous chemiluminescence probes for other enzymes relevant to specific bacteria detection and point‐of‐care diagnostics.
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