AmDREB2C, from Ammopiptanthus mongolicus, enhances abiotic stress tolerance and regulates fatty acid composition in transgenic Arabidopsis

拟南芥 生物 非生物胁迫 多不饱和脂肪酸 转基因 植物 非生物成分 常绿 耐旱性 基因 脂肪酸 生物化学 生态学 突变体
作者
Yumei Yin,Xiaoxu Jiang,Meiyan Ren,Min Xue,Dina Nan,Zhilin Wang,Yanping Xing,Maoyan Wang
出处
期刊:Plant Physiology and Biochemistry [Elsevier]
卷期号:130: 517-528 被引量:23
标识
DOI:10.1016/j.plaphy.2018.08.002
摘要

Dehydration-responsive element-binding (DREB) transcription factors (TFs) play a vital role in plant response to abiotic stresses. However, little is known about DREB TFs in plants adapted to harsh environments and in the formation of polyunsaturated fatty acids (PUFAs), a major membrane component closely associated with plant stress tolerance. Here, we characterized AmDREB2C in Ammopiptanthus mongolicus (Maxim. ex kom.) Cheng F., a desert evergreen broadleaf shrub with a high tolerance to harsh environments. AmDREB2C encodes a canonical DREB2-type TF, and the protein was localized in the nucleus. AmDREB2C had the highest expression levels in leaves of naturally growing shrubs in the wild during the winter season of a year of sampling. The expression was also induced by cold, heat and drought stresses in laboratory-cultured seedlings. Moreover, AmDREB2C was most abundantly expressed in young leaves and immature seeds rather than other tissues of the shrubs. Constitutive expression of AmDREB2C in Arabidopsis enhanced freezing, heat and drought tolerances of the transgenic plants, likely through inducing the expression of important stress-responsive genes. The transgene also increased the level of linolenic acid (C18:3), a major PUFA in most plant species, in leaves and seeds of the transgenic plants. Correspondingly, the transcription of FAD3, FAD7 and FAD8, three genes encoding fatty acid desaturases (FADs) responsible for the production of C18:3, showed a differential up-regulation in these two organs. This study provides new insight into the underlying molecular mechanisms of A. mongolicus' ability to endure harsh environments and DREB TF regulation of fatty acid desaturation.
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