ENZYME MARKERS ACTIVITY AND BILE FORMATION FUNCTION OF LIVER IN CASES OF TUBERCULOSTATICS AND HEXAVALENT CHROMIUM COMPOUNDS AFFECTION IN RATS

<p>Background. Currently, the growing incidence of toxic lesions of the liver is associated with<br />industrial chemicalization and uncontrolled use of hepatotoxic drugs in everyday life. There are about<br />one thousand drugs with high or low hepatotoxicity, such as anti-TB drugs.<br />Objective. In this research we studied the intracellular enzymes activity and bile formation function<br />of the liver in rats of different ages in cases of tuberculostatic (isoniazid and rifampicin) affection and<br />chromium (potassium dichromate) intoxication.<br />Methods. The experimental affection of rats of different ages was performed by combined injection<br />of hexavalent chromium compounds (a solution of potassium dichromate, 3 mg/kg), isoniazid<br />(0.05 g/kg) and rifampicin (0.25 g/kg). On the 7th and 14th days the rats were injected with enterosorbent<br />Sorbex (150 mg/kg). Enzyme markers activity of the liver was evaluated due to alanine and aspartate<br />aminotransferases (ALT and AST) and alkaline phosphatase (ALP) rates. Bile formation function of the<br />liver was evaluated by total bilirubin and bile acids content in blood.<br />Results. The disorders in hepatocytes plasma membranes permeability were defined by the<br />increased rates of ALT, AST and alkaline phosphatase in blood serum which were decreased in the<br />liver. It was determined that total bilirubin and bile acids content in blood serum of the affected<br />animals increased. It influenced hepatocytes excretion in bile capillaries and caused cholestasis and<br />revenues decrease in bile.<br />Conclusions. The most significant metabolic disorders in cases of chrome-isoniazid-rifampicin<br />affection were defined in immature and senior animals in comparison with mature animals.<br />KEY WORDS: isoniazid, rifampicin, hexavalent chromium compounds, liver enzymes,<br />bile formation.</p>