Adjusting the Linear Range of Au-MOF Fluorescent Probes for Real-Time Analyzing Intracellular GSH in Living Cells

A series of Au-loaded metal–organic frameworks (MOFs) were synthesized in this study and further employed for real-time quantitative analysis of intracellular glutathione (GSH) level. Different linear ranges can be acquired by altering the size of gold and MOF particles, or adjusting the proportion of 2-aminoterephthalic acid/1,4-benzenedicarboxylate linkers, which is also observed on fluorescein isothiocyanate-attached Au-MOFs. Further study reveals that the flexible molecular chain of GSH with the −COOH/–NH2 and −SH terminals may readily tie on relevant gold nanoparticles through its −NH2/–COOH groups, which then restricts the intramolecular motions of fluorescence probes and thus induces marked fluorescence enhancement. On the basis of these observations, the intracellular GSH levels of different cells including L02 cells, Hela, and U87 as well as HepG2 cancer cells can be rapidly evaluated by these Au-MOF probes.