Micropatterned Surfaces for Control of Cell Shape, Position, and Function

Biotechnology ProgressVolume 14, Issue 3 p. 356-363 Article Micropatterned Surfaces for Control of Cell Shape, Position, and Function Christopher S. Chen, Christopher S. Chen Departments of Surgery and Pathology, Children's Hospital and Harvard Medical School, Enders 1007, 300 Longwood Avenue, Boston, Massachusetts 02115Search for more papers by this authorMilan Mrksich, Milan Mrksich Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138Search for more papers by this authorSui Huang, Sui Huang Departments of Surgery and Pathology, Children's Hospital and Harvard Medical School, Enders 1007, 300 Longwood Avenue, Boston, Massachusetts 02115Search for more papers by this authorGeorge M. Whitesides, George M. Whitesides Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138Search for more papers by this authorDonald E. Ingber, Corresponding Author Donald E. Ingber ingber@a1.tch.harvard.edu Departments of Surgery and Pathology, Children's Hospital and Harvard Medical School, Enders 1007, 300 Longwood Avenue, Boston, Massachusetts 02115Departments of Surgery and Pathology, Children's Hospital and Harvard Medical School, Enders 1007, 300 Longwood Avenue, Boston, Massachusetts 02115. Telephone: 617–355–8031. Fax: 617–232–7914.===Search for more papers by this author Christopher S. Chen, Christopher S. Chen Departments of Surgery and Pathology, Children's Hospital and Harvard Medical School, Enders 1007, 300 Longwood Avenue, Boston, Massachusetts 02115Search for more papers by this authorMilan Mrksich, Milan Mrksich Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138Search for more papers by this authorSui Huang, Sui Huang Departments of Surgery and Pathology, Children's Hospital and Harvard Medical School, Enders 1007, 300 Longwood Avenue, Boston, Massachusetts 02115Search for more papers by this authorGeorge M. Whitesides, George M. Whitesides Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138Search for more papers by this authorDonald E. Ingber, Corresponding Author Donald E. Ingber ingber@a1.tch.harvard.edu Departments of Surgery and Pathology, Children's Hospital and Harvard Medical School, Enders 1007, 300 Longwood Avenue, Boston, Massachusetts 02115Departments of Surgery and Pathology, Children's Hospital and Harvard Medical School, Enders 1007, 300 Longwood Avenue, Boston, Massachusetts 02115. Telephone: 617–355–8031. Fax: 617–232–7914.===Search for more papers by this author First published: 05 September 2008 https://doi.org/10.1021/bp980031mCitations: 588Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract The control of cell position and function is a fundamental focus in the development of applications ranging from cellular biosensors to tissue engineering. Using microcontact printing of self-assembled monolayers (SAMs) of alkanethiolates on gold, we manufactured substrates that contained micrometer-scale islands of extracellular matrix (ECM) separated by nonadhesive regions such that the pattern of islands determined the distribution and position of bovine and human endothelial cells. In addition, the size and geometry of the islands were shown to control cell shape. Traditional approaches to modulate cell shape, either by attaching suspended cells to microbeads of different sizes or by plating cells on substrates coated with different densities of ECM, suggested that cell shape may play an important role in control of apoptosis as well as growth. Data are presented which show how micropatterned substrates were used to definitively test this hypothesis. Progressively restricting bovine and human endothelial cell extension by culturing cells on smaller and smaller micropatterned adhesive islands regulated a transition from growth to apoptosis on a single continuum of cell spreading, thus confirming the central role of cell shape in cell function. The micropatterning technology is therefore essential not only for construction of biosurface devices but also for the investigation of the fundamental biology of cell−ECM interactions. Citing Literature Volume14, Issue31998Pages 356-363 RelatedInformation