GCLC公司
小发夹RNA
细胞生长
GCLM公司
下调和上调
化学
基因敲除
谷胱甘肽
癌症研究
莱菔硫烷
分子生物学
细胞凋亡
生物
生物化学
基因
酶
作者
Chan Ho Jang,Jinkyung Lee,Min Jeong Kwon,Yoonsu Kim,Ji Hyeong Chae,Yoon Ah Jeong,Eun Jeong Kwon,Jisun Oh,Jong‐Sang Kim
标识
DOI:10.1096/fasebj.2022.36.s1.r4214
摘要
Sulforaphane (SFN), a sulfur-containing compound that belongs to the isothiocyanate class of dietary phytochemicals found in cruciferous vegetables, has been known to cause a hormetic effect on cancer cell growth. We previously reported that SFN at low doses stimulated proliferation of HCT116 human colorectal carcinoma cells through Nrf2 activation in vitro and in vivo. Recently, we discovered that brusatol, a nuclear factor erythroid 2-related factor 2 (Nrf2) inhibitor, significantly suppressed the SFN-induced proliferation of HCT116 cells. Therefore, we hypothesized that SFN-induced proliferation of HCT116 cells is mediated by an Nrf2 downstream gene(s). Transcriptomic profiling revealed a set of differentially expressed genes (DEGs) involved in glutathione (GSH) metabolism as compared between wildtype (WT) and Nrf2 knockout (KO) HCT116 cells treated with or without SFN. In particular, we found that glutamate-cysteine ligase catalytic subunit (GCLC) was highly elevated in SFN-treated WT HCT116 cells among the DEGs, but not in SFN-treated Nrf2 KO HCT116 cells. To confirm if upregulation of γ-glutamate-cysteine ligase (γ-GCL) is associated with SFN-induced proliferation of HCT116 cells, inhibiting γ-GCL by buthionine sulfoximine (BSO, a selective γ-GCL inhibitor) and silencing GCLC by short hairpin RNA (shRNA) were carried out. The results showed that SFN-induced proliferation of HCT116 cells was significantly reduced by γ-GCL inhibition and abolished by GCLC knockdown. Furthermore, we confirmed that BSO suppressed SFN-induced tumor growth of WT HCT116 xenograft mice and the tumor growth was not stimulated in both Nrf2 KO and GCLC shRNA HCT16 xenograft mice by SFN at a low dose. Collectively, these findings indicate that upregulation of GCLC-dependent GSH biosynthesis by SFN promotes proliferation of HCT116 cells and thus GCL could be a potential target for chemotherapy.
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