Resveratrol Promotes Osteogenic Differentiation of Canine Bone Marrow Mesenchymal Stem Cells Through Wnt/Beta-Catenin Signaling Pathway

Bone marrow mesenchymal stem cells (BMSCs) can transdifferentiate into different types of cells and may serve as a cell source for tissue engineering. Resveratrol has been shown to possess many benefits, including activation of osteogenesis. Furthermore, Wnt/β-catenin signaling has been known to promote osteogenic differentiation in many cells. In this study, we investigated the role of resveratrol in osteoblastic differentiation of canine BMSCs. Resveratrol treatment of canine BMSCs remarkably increased alkaline phosphatase activity and calcium nodules, inhibited the function of glycogen synthase kinase 3β, led to an increase in stabilization and nuclear accumulation of β-catenin, and upregulated expression of osteoblast-related marker gene expression. In addition, resveratrol caused rapid activation of ERK1/2. Collectively, our results indicate that resveratrol promotes osteoblastic differentiation of canine BMSCs by activating the Wnt/β-catenin; ERK/MAPK signaling pathways are also involved in osteogenic differentiation of canine BMSCs.