肺癌
支气管肺泡灌洗
唾液
小桶
生物
癌症
微生物群
肺
癌
病理
免疫学
医学
内科学
生物信息学
基因
遗传学
转录组
基因表达
作者
Ke Wang,Yufen Huang,Zhenqiang Zhang,Jinling Liao,Yudi Ding,Xiaodong Fang,Lihua Liu,Jing Luo,Jinliang Kong
出处
期刊:Medical Science Monitor
[International Scientific Information, Inc.]
日期:2019-04-17
卷期号:25: 2819-2834
被引量:47
摘要
BACKGROUND:The present study aimed to evaluate the difference in microbiota diversity in the oral cavity and fluid bronchoalveolar lavage (BALF) of patients with lung cancer. MATERIAL AND METHODS:Buccal (saliva) and lower respiratory tract BALF samples were collected from 51 patients with primary bronchogenic carcinoma and 15 healthy controls, and bacterial genomic DNA was extracted. High-throughput 16S rDNA amplicon sequencing was performed, and microbial diversity, composition, and functions of microbiota were analyzed by bioinformatics methods. RESULTS:Patients with lung cancer have lower microbial diversity than healthy controls in both saliva and BALF samples. Significant segregation was observed between the different pathological types of lung cancer groups and the control group regardless of the sampling site. Treponema and Filifactor were identified as potential bacterial biomarkers in BALF samples, while Filifactor was ideal to distinguish healthy controls from lung cancer patients. Moreover, the predictive variation analysis of the KEGG (Kyoto Encyclopedia of Genes and Genomes) metabolic pathway showed that the metabolic differences in microbiota varied by sampling site. CONCLUSIONS:Lung cancer patients carry a different and less diverse microorganism community than healthy controls. Certain bacterial taxa might be associated with lung cancer, but the exact species depends on the sampling site and the pathological type. This study provides basic data on the microbiota diversity in BALF and saliva samples from lung cancer patients. Further investigation with a larger sample size should help validate the enriched species in different pathological types of lung cancers.
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