Environmental pollution and its consequent biological toxicity present formidable challenges in the contemporary world, amidst the endeavor to harness environmentally friendly materials as an effective strategy. Molybdenum disulfide (MoS2), a highly esteemed graphene-like nanomaterial, stands out as a prominent candidate owing to its outstanding properties, abundant availability, and promising development prospects. Its inherent biocompatibility not only holds immense potential for environmental waste-water treatment but also exhibits extraordinary promise in the realm of biotechnology, such as bactericidal and tumor therapy. The progression of typical MoS2 applications, from water treatment to tumor treatment, is reviewed, with a specific emphasis on the innovative utilization of MoS2 in these dual scenarios. In addition, we also anticipate the future applications of MoS2 based on cost-recovery efficiency.