斑点图案
相(物质)
化学
细胞生物学
生物物理学
生物
计算机科学
人工智能
有机化学
作者
Mengjun Zhang,Zhuang Gu,Shuanghui Guo,Yingtian Sun,Suibin Ma,Shuo Yang,Jierui Guo,Chenxi Fang,Shu Li,Yifan Ge,Zhongwen Chen,Bo Wang
出处
期刊:Cell Reports
[Elsevier]
日期:2024-02-20
卷期号:43 (3): 113827-113827
被引量:7
标识
DOI:10.1016/j.celrep.2024.113827
摘要
Nuclear speckles (NSs) are nuclear biomolecular condensates that are postulated to form by macromolecular phase separation, although the detailed underlying forces driving NS formation remain elusive. SRRM2 and SON are 2 non-redundant scaffold proteins for NSs. How each individual protein governs assembly of the NS protein network and the functional relationship between SRRM2 and SON are largely unknown. Here, we uncover immiscible multiphases of SRRM2 and SON within NSs. SRRM2 and SON are functionally independent, specifically regulating alternative splicing of subsets of mRNA targets, respectively. We further show that SRRM2 forms multicomponent liquid phases in cells to drive NS subcompartmentalization, which is reliant on homotypic interaction and heterotypic non-selective protein-RNA complex coacervation-driven phase separation. SRRM2 serine/arginine-rich (RS) domains form higher-order oligomers and can be replaced by oligomerizable synthetic modules. The serine residues within the RS domains, however, play an irreplaceable role in fine-tuning the liquidity of NSs.
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