脾后皮质
消光(光学矿物学)
神经科学
松弛素
调制(音乐)
心理学
皮质(解剖学)
受体
生物
医学
内科学
物理
古生物学
声学
作者
Mónica Navarro-Sánchez,Isis Gil‐Miravet,Daniel Montero-Caballero,Ross A. D. Bathgate,Mohamed Akhter Hossain,Esther Castillo-Gómez,Andrew L. Gundlach,Francisco E. Olucha‐Bordonau
摘要
The retrosplenial cortex (RSC) plays a central role in processing contextual fear conditioning. In addition to corticocortical and thalamocortical projections, the RSC receives subcortical inputs, including a substantial projection from the nucleus incertus in the pontine tegmentum. This GABAergic projection contains the neuropeptide, relaxin-3 (RLN3), which inhibits target neurons via its Gi/o-protein-coupled receptor, RXFP3. To assess this peptidergic system role in contextual fear conditioning we bilaterally injected adult rats RSC with an adeno-associated-virus (AAV), expressing the chimeric RXFP3 agonist R3/I5 or a control AAV, and subjected them to contextual fear conditioning. The R3/I5 injected rats did not display any differences compared to control-injected and naïve rats, but displayed a significantly delayed extinction. Subsequently, we employed acute bilateral injections of the specific RXFP3 agonist peptide, RXFP3-Analogue 2 (A2), into RSC. While the administration of A2 before each extinction trial showed no impact on the extinction process, treatment with A2 before each acquisition trial resulted in delayed extinction. In related anatomical studies, we detected an enrichment of RLN3-immunoreactive nerve fibers in deep layers of the RSC, and a higher level of co-localization of RXFP3 mRNA with vesicular GABA transporter (vGAT) mRNA than with vesicular glutamate transporter-1 (vGLUT1) mRNA across the RSC, consistent with an effect of RLN3/RXFP3 signalling on the intrinsic, inhibitory circuits within the RSC. These findings suggest that contextual conditioning processes in the RSC involve, in part, RLN3 afferent activation of local inhibitory neurons that provides a stronger memory acquisition which, in turn, retards the extinction process.
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