清晨好,您是今天最早来到科研通的研友!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您科研之路漫漫前行!

Massively parallel characterization of regulatory elements in the developing human cortex

增强子 巨量平行 调节顺序 染色质 类有机物 基因 报告基因 基因表达调控 计算生物学 计算机科学 皮质激素生成 细胞生物学 生物 神经科学 转录因子 基因表达 遗传学 胚胎干细胞 并行计算
作者
Chengyu Deng,Sean Whalen,Marilyn Steyert,Ryan Ziffra,Pawel F. Przytycki,Fumitaka Inoue,Daniela A. Pereira,Davide Capauto,Scott Norton,Flora M. Vaccarino,Alex A. Pollen,Tomasz J. Nowakowski,Nadav Ahituv,Katherine S. Pollard,Schahram Akbarian,Alexej Abyzov,Nadav Ahituv,Dhivya Arasappan,José Juan Almagro Armenteros,Brian J. Beliveau,Jaroslav Bendl,Sabina Berretta,Rahul Bharadwaj,Arjun Bhattacharya,Lucy Bicks,Kristen Brennand,Davide Capauto,Frances A. Champagne,Tanima Chatterjee,Chris Chatzinakos,Yuhang Chen,H. Isaac Chen,Yuyan Cheng,Lijun Cheng,Andrew Chess,Jo-fan Chien,Zhiyuan Chu,Declan Clarke,Ashley Clement,Leonardo Collado‐Torres,Gregory M. Cooper,Gregory E. Crawford,Rujia Dai,Nikolaos P. Daskalakis,José Dávila-Velderrain,Amy Deep‐Soboslay,Chengyu Deng,Christopher P. DiPietro,Stella Dracheva,Shiron Drusinsky,Ziheng Duan,Duc M. Duong,Cagatay Dursun,Nicholas J. Eagles,Jonathan I. Edelstein,Prashant S. Emani,John F. Fullard,Kiki Galani,Timur R. Galeev,Michael J. Gandal,Sophia C. Gaynor,Mark Gerstein,Daniel H. Geschwind,Kiran Girdhar,Fernando S. Goes,William J. Greenleaf,Jennifer Grundman,Hanmin Guo,Qiuyu Guo,Chirag Gupta,Yoav Hadas,Joachim Hallmayer,Xikun Han,Vahram Haroutunian,Natalie Hawken,Chuan He,Ella Henry,Stephanie C. Hicks,Marcus Ho,Li‐Lun Ho,Gabriel E. Hoffman,Yi‐Ling Huang,Louise A. Huuki-Myers,Ahyeon Hwang,Thomas M. Hyde,Artemis Iatrou,Fumitaka Inoue,Aarti Jajoo,Matthew L. Jensen,Lihua Jiang,Jin Peng,Ting Jin,Connor Jops,Alexandre Jourdon,Riki Kawaguchi,Manolis Kellis,Saniya Khullar,Joel E. Kleinman,Steven P. Kleopoulos,Alexey Kozlenkov,Arnold R. Kriegstein,Anshul Kundaje,Soumya Kundu,Cheyu Lee,Donghoon Lee,Junhao Li,Mingfeng Li,Xiao Lin,Shuang Liu,Jason Liu,Jianyin Liu,Chunyu Liu,Shuang Liu,Shaoke Lou,Jacob M. Loupe,Dan Lü,Shaojie Ma,Liang Ma,Michael Margolis,Jessica Mariani,Keri Martinowich,Kristen R. Maynard,Samantha Mazariegos,Ran Meng,Richard M. Myers,Courtney Micallef,Tatiana Mikhailova,Guo‐li Ming,Shahin Mohammadi,Emma Monte,Kelsey S. Montgomery,Jill E. Moore,Jennifer Moran,Eran A. Mukamel,Angus C. Nairn,Charles B. Nemeroff,Pengyu Ni,Scott Norton,Tomasz J. Nowakowski,Larsson Omberg,Stephanie C. Page,Saejeong Park,Ashok Patowary,Reenal Pattni,Geo Pertea,Mette A. Peters,Nishigandha Phalke,Dalila Pinto,Milos Pjanic,Sirisha Pochareddy,Katherine S. Pollard,Alex A. Pollen,Henry Pratt,Pawel F. Przytycki,Carolin Purmann,Zhaohui Qin,Pingping Qu,Diana Quintero,Towfique Raj,Ananya S. Rajagopalan,Sarah Reach,Thomas Reimonn,Kerry J. Ressler,D D Ross,Panos Roussos,Joel Rozowsky,Misir Ruth,W. Brad Ruzicka,Stephan Sanders,Juliane M. Schneider,Soraya Scuderi,Robert Sebra,Nenad Šestan,Nicholas T. Seyfried,Zhiping Shao,Nicole Shedd,Annie W. Shieh,Joo Heon Shin,Mario Škarica,Clara Snijders,Hongjun Song,Matthew W. State,Jason L. Stein,Marilyn Steyert,Sivan Subburaju,Thomas C. Südhof,M Snyder,Ran Tao,Karen Therrien,Li‐Huei Tsai,Alexander E. Urban,Flora M. Vaccarino,Harm van Bakel,Daniel Vo,Georgios Voloudakis,Brie Wamsley,Tao Wang,Sidney H. Wang,Daifeng Wang,Yifan Wang,Jonathan Warrell,Wei Yu,Annika K. Weimer,Daniel R. Weinberger,Cindy Wen,Zhiping Weng,Sean Whalen,Kevin P. White,A. Jeremy Willsey,Hyejung Won,Wing H. Wong,Hao Wu,Feinan Wu,Stefan Wuchty,Dennis Wylie,Siwei Xu,Chloe X. Yap,Biao Zeng,Pan Zhang,Chunling Zhang,Bin Zhang,Jing Zhang,Yanqiong Zhang,Xiao Zhou,Ryan Ziffra,Zane Zeier,Trisha M. Zintel
出处
期刊:Science [American Association for the Advancement of Science (AAAS)]
卷期号:384 (6698) 被引量:6
标识
DOI:10.1126/science.adh0559
摘要

Nucleotide changes in gene regulatory elements are important determinants of neuronal development and diseases. Using massively parallel reporter assays in primary human cells from mid-gestation cortex and cerebral organoids, we interrogated the cis-regulatory activity of 102,767 open chromatin regions, including thousands of sequences with cell type–specific accessibility and variants associated with brain gene regulation. In primary cells, we identified 46,802 active enhancer sequences and 164 variants that alter enhancer activity. Activity was comparable in organoids and primary cells, suggesting that organoids provide an adequate model for the developing cortex. Using deep learning we decoded the sequence basis and upstream regulators of enhancer activity. This work establishes a comprehensive catalog of functional gene regulatory elements and variants in human neuronal development.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
15秒前
20秒前
斯文败类应助帮帮我好吗采纳,获得10
38秒前
46秒前
1250241652完成签到,获得积分10
46秒前
研友_nxw2xL完成签到,获得积分10
50秒前
muriel完成签到,获得积分10
57秒前
科研通AI2S应助帮帮我好吗采纳,获得10
1分钟前
苗条翠阳完成签到 ,获得积分10
1分钟前
文瑄完成签到 ,获得积分10
2分钟前
深情安青应助帮帮我好吗采纳,获得10
2分钟前
ly完成签到 ,获得积分10
2分钟前
2分钟前
3分钟前
善良冷松发布了新的文献求助10
3分钟前
俊逸吐司完成签到 ,获得积分10
3分钟前
研友_LmgOaZ完成签到 ,获得积分0
3分钟前
善良冷松完成签到,获得积分20
3分钟前
4分钟前
4分钟前
4分钟前
4分钟前
陈无敌完成签到 ,获得积分10
4分钟前
Richard完成签到 ,获得积分10
5分钟前
顾矜应助帮帮我好吗采纳,获得10
5分钟前
CC完成签到 ,获得积分10
6分钟前
6分钟前
6分钟前
6分钟前
6分钟前
7分钟前
7分钟前
开放访天完成签到 ,获得积分10
7分钟前
7分钟前
炫哥IRIS完成签到,获得积分10
8分钟前
8分钟前
路在脚下完成签到 ,获得积分10
8分钟前
不回首完成签到 ,获得积分10
8分钟前
9分钟前
Owen应助帮帮我好吗采纳,获得10
9分钟前
高分求助中
Sustainability in Tides Chemistry 2800
The Young builders of New china : the visit of the delegation of the WFDY to the Chinese People's Republic 1000
Rechtsphilosophie 1000
Bayesian Models of Cognition:Reverse Engineering the Mind 888
Defense against predation 800
Very-high-order BVD Schemes Using β-variable THINC Method 568
Chen Hansheng: China’s Last Romantic Revolutionary 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3137028
求助须知:如何正确求助?哪些是违规求助? 2787992
关于积分的说明 7784214
捐赠科研通 2444073
什么是DOI,文献DOI怎么找? 1299719
科研通“疑难数据库(出版商)”最低求助积分说明 625513
版权声明 600997