生物
脆弱类杆菌
拟杆菌
微生物学
人体微生物群
细菌
寄主(生物学)
基因组
微生物群
共生
遗传学
基因
作者
Juntao Shen,Jieqiong Zhang,Luofei Mo,Yanchen Li,Yake Li,Cun Li,Xiaoxian Kuang,Zining Tao,Zepeng Qu,Lu Wu,Junyu Chen,Shiying Liu,Linfang Zeng,Zexi He,Zuohong Chen,Yu Deng,Tong Zhang,Bing Li,Lei Dai,Yingfei Ma
标识
DOI:10.1016/j.chom.2023.03.013
摘要
Phages are highly abundant in the human gut, yet most of them remain uncultured. Here, we present a gut phage isolate collection (GPIC) containing 209 phages for 42 commensal human gut bacterial species. Genome analysis of the phages identified 34 undescribed genera. We discovered 22 phages from the Salasmaviridae family that have small genomes (∼10-20 kbp) and infect Gram-positive bacteria. Two phages from a candidate family, Paboviridae, with high prevalence in the human gut were also identified. Infection assays showed that Bacteroides and Parabacteroides phages are specific to a bacterial species, and strains of the same species also exhibit substantial variations in phage susceptibility. A cocktail of 8 phages with a broad host range for Bacteroides fragilis strains effectively reduced their abundance in complex host-derived communities in vitro. Our study expands the diversity of cultured human gut bacterial phages and provides a valuable resource for human microbiome engineering.
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