Enhancing laser speckle contrast imaging based on adaptive scale and directional kernel during V-PDT

Laser Speckle Contrast Imaging (LSCI) as the advantages of high speed, wide field of view, and ease of implementation has been widely used for imaging blood flow and vascular deformation during Vascular targeted Photodynamic Therapy (V-PDT). However, uncorrected direction and or scale kernel failed to improve the visualization of blood vessels derived from poor Signal Noise Ratio (SNR) of micro vessels during V-PDT. In this study, we extracted the adaptive scale and directional kernel using dual-wavelength reflection imaging to enhance laser speckle imaging. The in vivo data showed that the proposed method improved the 4%-29% performance of contrast-to-noise ratio (CNR) and provide a powerful monitoring tool for blood flow and vascular deformation during V-PDT.