脚手架
壳聚糖
生物相容性
复合数
组织工程
材料科学
粘附
生物医学工程
骨组织
微观结构
纳米技术
化学
复合材料
生物化学
医学
冶金
作者
Wenmin Tang,Peng Pan,Tiantian Chen,Jian Wang,Xinyan Tracy Cui,Wentao Liu,Liang Kan
标识
DOI:10.1016/j.colsurfb.2024.114199
摘要
Bone defect has always been a difficult problem in clinical work. According to the current research results, tissue engineered scaffolds with a single function, structure, and composition are not sufficient to repair complex bone defects. In this work, a three-dimensional (3D) chitosan degradable composite scaffold loaded with zinc oxide (ZnO) was constructed, and the effect of ZnO content on scaffold performance and osteogenesis was explored. The 3D composite scaffold was prepared by freeze-drying technology. The microstructure, porosity, degradation performance, release performance, swelling performance, cytotoxicity, cell adhesion and osteogenic ability of ZnO nanoparticles and chitosan (ZnONPs/CS) composite scaffolds were measured. The results show that an appropriate amount of ZnO may be helpful to regulate the stability and degradation characteristics of the scaffold to a certain extent. Moreover, the composite scaffold could release ZnO into the simulated body fluid environment. The appropriate amount of ZnO helps to promote the proliferation, adhesion, and osteogenic differentiation of MC3T3-E1 cells. At a ZnO content of 3 wt%, both in vitro and vivo results showed relatively optimal biocompatibility and bioactivity of the scaffolds. This work could at least provide some positive insights for the selection of ZnO dosage, construction of chitosan-based 3D scaffolds, tissue engineering applications, and clinical treatment.
科研通智能强力驱动
Strongly Powered by AbleSci AI