The MUC13 cell-surface mucin protects against intestinal inflammation by inhibiting epithelial cell apoptosis

细胞凋亡 炎症 结肠炎 肠粘膜 免疫学 粘蛋白 医学 癌症研究 炎症性肠病 肠上皮 程序性细胞死亡 生物 上皮 病理 内科学 疾病 生物化学
作者
Yonghua Sheng,Rohan Lourie,Sara K. Lindén,Penny L. Jeffery,Deborah Roche,Tammy Tran,Chin Wen Png,Nigel J. Waterhouse,Philip Sutton,Timothy H. Florin,Michael A. McGuckin
出处
期刊:Gut [BMJ]
卷期号:60 (12): 1661-1670 被引量:117
标识
DOI:10.1136/gut.2011.239194
摘要

The MUC13 transmembrane mucin is highly and constitutively expressed in the small and large intestine. Although MUC13 polymorphisms have been associated with human inflammatory bowel diseases and susceptibility to Escherichia coli infection in pigs, the biological functions of MUC13 are unknown. This study aimed to explore whether MUC13 modulates intestinal inflammation.Muc13(-/-) mice were generated, phenotyped and challenged with the colitis-inducing agent, dextran sodium sulphate (DSS). Colitis was assessed by clinical symptoms and intestinal histopathology. Intestinal epithelial cell apoptosis and proliferation, macrophage infiltration and cytokine production were also quantified. Apoptosis of human LS513 intestinal epithelial cells in response to apoptotic agents, including DSS, was also measured, following knockdown of MUC13 with siRNA.Muc13(-/-) mice were viable, fertile and developed normally, with no spontaneous intestinal pathology except mild focal neutrophilic inflammation in the small and large intestines of old mice. In response to DSS challenge, Muc13(-/-) mice developed more severe acute colitis, as reflected by increased weight loss, rectal bleeding, diarrhoea and histological colitis scores compared with wild-type mice. Increased numbers of F4/80(+) macrophages in inflamed mucosa of Muc13(-/-) mice were accompanied by increased expression of intestinal IL-1β and TNFα mRNA. Muc13(-/-) mice had significantly increased intestinal epithelial cell apoptosis within 3 days of DSS exposure. LS513 cells were more susceptible to DSS, actinomycin-D, ultraviolet irradiation and TRAIL-induced apoptosis when MUC13 was knocked down by siRNA.These novel findings indicate a protective role for Muc13 in the colonic epithelium by inhibiting toxin-induced apoptosis and have important implications for intestinal infections, inflammatory diseases and the development of intestinal cancer.
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