口腔链球菌
生物
遗传学
葡萄糖基转移酶
基因
抑制消减杂交
微生物学
链球菌
细菌
肽序列
cDNA文库
作者
H.K. Park,H.J. Lee,Eun-Gyeong Jeong,Hyoung‐Shik Shin,Wangdo Kim
标识
DOI:10.1177/0022034510378426
摘要
Although the pathogenesis of Streptococcus oralis may be different from that of other viridans group streptococci, S. oralis shares a high degree of DNA sequence similarity with these streptococci. As a result, discrimination of S. oralis from its close relatives has long been considered difficult. This study was conducted to find specific genes that allow for the in vitro identification of S. oralis, but not other oral commensals. Four hundred ninety S. oralis clones obtained by suppressive subtractive hybridization were used for Southern hybridization, and positive clones were sequenced. Of 5 S. oralis-specific clones, newly designed primer sets based on the glucosyltransferase regulatory gene amplified genomic DNA only from S. oralis strains, but not from any of the other 125 strains tested. Our findings may be useful for the future development of efficient diagnostic tools for the rapid identification and differentiation of S. oralis from other oral streptococci strains.
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